Rotavirus G and P genotypes in rural Ghana

Richard H. Asmah; Jon Green; George E. Armah; Chris I. Gallimore; Jim J. Gray; Miren Iturriza-Gómara; Francis Anto; Abraham Oduro; Fred N. Binka; David W. G. Brown; Felicity Cutts

doi:10.1128/JCM.39.5.1981-1984.2001

Rotavirus G and P genotypes in rural Ghana

Richard H. Asmah, Jon Green, George E. Armah, Chris I. Gallimore, Jim J. Gray, Miren Iturriza-Gómara, Francis Anto, Abraham Oduro, Fred N. Binka, David W. G. Brown, Felicity Cutts

Norwich Medical School

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Abstract

An epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural Upper Eastern Region of Ghana during 1998. Fifty isolates, randomly selected from 165 human group A rotavirus-positive samples, were G and P characterized by a reverse transcription (RT)-PCR assay using a seminested multiplex method. Rotaviruses of the G3 genotype were found to be the predominant strain (78%), followed by G2 (14%) and G1 (2%). Mixed infections, as shown by combinations of G3 and G2 (4%) and G3 and G1 (2%), were also observed. P typing showed P[4] (72.34%) to be the prevalent strain, followed by P[6] (21.3%), P[8] (2.13%), and a combination of P[4] and P[6] (4.3%).

Original language	English
Pages (from-to)	1981-1984
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	39
Issue number	5
DOIs	https://doi.org/10.1128/JCM.39.5.1981-1984.2001
Publication status	Published - 2001

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10.1128/JCM.39.5.1981-1984.2001

Cite this

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title = "Rotavirus G and P genotypes in rural Ghana",

abstract = "An epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural Upper Eastern Region of Ghana during 1998. Fifty isolates, randomly selected from 165 human group A rotavirus-positive samples, were G and P characterized by a reverse transcription (RT)-PCR assay using a seminested multiplex method. Rotaviruses of the G3 genotype were found to be the predominant strain (78%), followed by G2 (14%) and G1 (2%). Mixed infections, as shown by combinations of G3 and G2 (4%) and G3 and G1 (2%), were also observed. P typing showed P[4] (72.34%) to be the prevalent strain, followed by P[6] (21.3%), P[8] (2.13%), and a combination of P[4] and P[6] (4.3%).",

author = "Asmah, {Richard H.} and Jon Green and Armah, {George E.} and Gallimore, {Chris I.} and Gray, {Jim J.} and Miren Iturriza-G{\'o}mara and Francis Anto and Abraham Oduro and Binka, {Fred N.} and Brown, {David W. G.} and Felicity Cutts",

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doi = "10.1128/JCM.39.5.1981-1984.2001",

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TY - JOUR

T1 - Rotavirus G and P genotypes in rural Ghana

AU - Asmah, Richard H.

AU - Green, Jon

AU - Armah, George E.

AU - Gallimore, Chris I.

AU - Gray, Jim J.

AU - Iturriza-Gómara, Miren

AU - Anto, Francis

AU - Oduro, Abraham

AU - Binka, Fred N.

AU - Brown, David W. G.

AU - Cutts, Felicity

PY - 2001

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N2 - An epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural Upper Eastern Region of Ghana during 1998. Fifty isolates, randomly selected from 165 human group A rotavirus-positive samples, were G and P characterized by a reverse transcription (RT)-PCR assay using a seminested multiplex method. Rotaviruses of the G3 genotype were found to be the predominant strain (78%), followed by G2 (14%) and G1 (2%). Mixed infections, as shown by combinations of G3 and G2 (4%) and G3 and G1 (2%), were also observed. P typing showed P[4] (72.34%) to be the prevalent strain, followed by P[6] (21.3%), P[8] (2.13%), and a combination of P[4] and P[6] (4.3%).

AB - An epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural Upper Eastern Region of Ghana during 1998. Fifty isolates, randomly selected from 165 human group A rotavirus-positive samples, were G and P characterized by a reverse transcription (RT)-PCR assay using a seminested multiplex method. Rotaviruses of the G3 genotype were found to be the predominant strain (78%), followed by G2 (14%) and G1 (2%). Mixed infections, as shown by combinations of G3 and G2 (4%) and G3 and G1 (2%), were also observed. P typing showed P[4] (72.34%) to be the prevalent strain, followed by P[6] (21.3%), P[8] (2.13%), and a combination of P[4] and P[6] (4.3%).

U2 - 10.1128/JCM.39.5.1981-1984.2001

DO - 10.1128/JCM.39.5.1981-1984.2001

M3 - Article

VL - 39

SP - 1981

EP - 1984

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

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ER -