Saturation transfer difference (STD) NMR spectroscopy characterization of dual binding mode of a mannose disaccharide to DC-SIGN

Jesus Angulo, Irene Díaz, Jose J. Reina, Georges Tabarani, Franck Fieschi, Javier Rojo, Pedro M. Nieto

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48 Citations (Scopus)

Abstract

Concurrent multiple binding modes of a single ligand can be detected and quantified by saturation transfer difference (STD) NMR spectroscopy. Analysis of experimental and predicted STD initial growing rates has allowed us to determine the precise orientation of Man a(1?2)Man in the minor complex with the carbohydrate recognition domain of DC-SIGN.
Original languageEnglish
Pages (from-to)2225-2227
Number of pages3
JournalChemBioChem
Volume9
Issue number14
DOIs
Publication statusPublished - 22 Sep 2008

Keywords

  • DC-SIGN
  • mannose
  • molecualr recognition
  • NMR spectroscopy
  • STD-NMR

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