Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci

Hiroshi Hisano, Brigid Meints, Matthew J. Moscou, Luis Cistue, Begoña Echávarri, Kazuhiro Sato, Patrick M. Hayes

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18 Citations (Scopus)
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Abstract

Barley (Hordeum vulgare) cv. ‘Golden Promise’ is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2 generation of immature embryos, derived from ‘Haruna Nijo’ × ‘Golden Promise,’ as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross ‘Golden Promise’ × ‘Full Pint.’ Based on SNP genotype, we selected lines having ‘Golden Promise’ alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to ‘Golden Promise.’ The results validate that the genetic substitution of TFA alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
Original languageEnglish
Pages (from-to)611-620
Number of pages10
JournalPlant Cell Reports
Volume36
Issue number4
Early online date15 Feb 2017
DOIs
Publication statusPublished - Apr 2017

Keywords

  • Agrobacterium tumefaciens
  • doubled haploid
  • Hordeum vulgare (barley)
  • single nucleotide polymorphism
  • transformation

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