Sensor NLR immune proteins activate oligomerization of their NRC helpers in response to plant pathogens

Mauricio P. Contreras, Hsuan Pai, Yasin Tumtas, Cian Duggan, Enoch Lok Him Yuen, Angel Vergara Cruces, Jiorgos Kourelis, Hee Kyung Ahn, Kim Teng Lee, Chih Hang Wu, Tolga O. Bozkurt, Lida Derevnina, Sophien Kamoun

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Nucleotide-binding domain leucine-rich repeat (NLR) immune receptors are important components of plant and metazoan innate immunity that can function as individual units or as pairs or networks. Upon activation, NLRs form multiprotein complexes termed resistosomes or inflammasomes. Although metazoan paired NLRs, such as NAIP/NLRC4, form hetero-complexes upon activation, the molecular mechanisms underpinning activation of plant paired NLRs, especially whether they associate in resistosome hetero-complexes, is unknown. In asterid plant species, the NLR required for cell death (NRC) immune receptor network is composed of multiple resistance protein sensors and downstream helpers that confer immunity against diverse plant pathogens. Here, we show that pathogen effector-activation of the NLR proteins Rx (confers virus resistance), and Bs2 (confers bacterial resistance) leads to oligomerization of their helper NLR, NRC2. Activated Rx does not oligomerize or enter into a stable complex with the NRC2 oligomer and remains cytoplasmic. In contrast, activated NRC2 oligomers accumulate in membrane-associated puncta. We propose an activation-and-release model for NLRs in the NRC immune receptor network. This points to a distinct activation model compared with mammalian paired NLRs.

Original languageEnglish
Article numbere111519
JournalEMBO Journal
Volume42
Issue number5
Early online date29 Dec 2022
DOIs
Publication statusPublished - Mar 2023

Keywords

  • immune receptor
  • inflammasome
  • NB-LRR
  • NOD-like receptor
  • resistosome

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