Siglec-F is a novel intestinal M cell marker

Nadezhda Gicheva, Matthew S. Macauley, Britni M. Arlian, James C. Paulson, Norihito Kawasaki

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Abstract

Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M-cells, demonstrating the potential to target M cells via Siglec-F.
Original languageEnglish
Pages (from-to)1–4
JournalBiochemical and Biophysical Research Communications
Volume479
Early online date11 Aug 2016
DOIs
Publication statusPublished - 7 Oct 2016

Keywords

  • Siglec
  • M cell
  • Oral vaccine
  • Peyer's patch

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