Simple synthesis of 32P-labelled inositol hexakisphosphates for study of phosphate transformations

Hayley Whitfield; Andrew M Riley; Soulla Diogenous; Himali Y Godage; Barry VL Potter; Charles A Brearley

doi:10.1007/s11104-017-3315-9

Simple synthesis of ³²P-labelled inositol hexakisphosphates for study of phosphate transformations

Hayley Whitfield, Andrew M Riley, Soulla Diogenous, Himali Y Godage, Barry VL Potter, Charles A Brearley (Lead Author)

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

17 Downloads (Pure)

Abstract

In many soils inositol hexakisphosphate in its various forms is as abundant
as inorganic phosphate. The organismal and geochemical processes that exchange phosphate between inositol hexakisphosphate and other pools of soil phosphate are poorly defined, as are the organisms and enzymes involved. We rationalized that simple enzymic synthesis of inositol hexakisphosphate labeled with 32P would
greatly enable study of transformation of soil inositol phosphates when combined with robust HPLC separations of different inositol phosphates.

Original language	English
Pages (from-to)	149–161
Journal	Plant and Soil
Volume	427
Issue number	1-2
Early online date	27 Jun 2017
DOIs	https://doi.org/10.1007/s11104-017-3315-9
Publication status	Published - Jun 2018

Keywords

Phytate
neo-
1D-chiro-
myo-
scyllo-inositol hexakisphosphate
Phosphate

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10.1007/s11104-017-3315-9Licence: CC BY

Published manuscript
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Final published version, 0.98 MBLicence: CC BY

Charles Brearley
- C.Brearleyuea.acuk
- School of Biological Sciences - Professor of Biochemistry
- Molecular Microbiology - Member
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching & Research

Phytases beyond phosphate, how inositol improves Feed Conversion ratio in poultry
Brearley, C. & Hajihosseini, M. K.
Biotechnology and Biological Sciences Research Council
27/06/16 → 26/11/18
Project: Research

Cite this

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title = "Simple synthesis of 32P-labelled inositol hexakisphosphates for study of phosphate transformations",

abstract = "In many soils inositol hexakisphosphate in its various forms is as abundantas inorganic phosphate. The organismal and geochemical processes that exchange phosphate between inositol hexakisphosphate and other pools of soil phosphate are poorly defined, as are the organisms and enzymes involved. We rationalized that simple enzymic synthesis of inositol hexakisphosphate labeled with 32P wouldgreatly enable study of transformation of soil inositol phosphates when combined with robust HPLC separations of different inositol phosphates.",

keywords = "Phytate, neo- , 1D-chiro-, myo-, scyllo-inositol hexakisphosphate, Phosphate",

author = "Hayley Whitfield and Riley, {Andrew M} and Soulla Diogenous and Godage, {Himali Y} and Potter, {Barry VL} and Brearley, {Charles A}",

year = "2018",

month = jun,

doi = "10.1007/s11104-017-3315-9",

language = "English",

volume = "427",

pages = "149–161",

journal = "Plant and Soil",

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T1 - Simple synthesis of 32P-labelled inositol hexakisphosphates for study of phosphate transformations

AU - Whitfield, Hayley

AU - Riley, Andrew M

AU - Diogenous, Soulla

AU - Godage, Himali Y

AU - Potter, Barry VL

AU - Brearley, Charles A

PY - 2018/6

Y1 - 2018/6

N2 - In many soils inositol hexakisphosphate in its various forms is as abundantas inorganic phosphate. The organismal and geochemical processes that exchange phosphate between inositol hexakisphosphate and other pools of soil phosphate are poorly defined, as are the organisms and enzymes involved. We rationalized that simple enzymic synthesis of inositol hexakisphosphate labeled with 32P wouldgreatly enable study of transformation of soil inositol phosphates when combined with robust HPLC separations of different inositol phosphates.

AB - In many soils inositol hexakisphosphate in its various forms is as abundantas inorganic phosphate. The organismal and geochemical processes that exchange phosphate between inositol hexakisphosphate and other pools of soil phosphate are poorly defined, as are the organisms and enzymes involved. We rationalized that simple enzymic synthesis of inositol hexakisphosphate labeled with 32P wouldgreatly enable study of transformation of soil inositol phosphates when combined with robust HPLC separations of different inositol phosphates.

KW - Phytate

KW - neo-

KW - 1D-chiro-

KW - myo-

KW - scyllo-inositol hexakisphosphate

KW - Phosphate

U2 - 10.1007/s11104-017-3315-9

DO - 10.1007/s11104-017-3315-9

M3 - Article

VL - 427

SP - 149

EP - 161

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1-2

ER -

Simple synthesis of 32P-labelled inositol hexakisphosphates for study of phosphate transformations

Abstract

Keywords

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Profiles

Charles Brearley

Projects

Phytases beyond phosphate, how inositol improves Feed Conversion ratio in poultry

Cite this

Simple synthesis of ³²P-labelled inositol hexakisphosphates for study of phosphate transformations