Simple synthesis of 32P-labelled inositol hexakisphosphates for study of phosphate transformations

Hayley Whitfield, Andrew M Riley, Soulla Diogenous, Himali Y Godage, Barry VL Potter, Charles A Brearley (Lead Author)

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Abstract

In many soils inositol hexakisphosphate in its various forms is as abundant
as inorganic phosphate. The organismal and geochemical processes that exchange phosphate between inositol hexakisphosphate and other pools of soil phosphate are poorly defined, as are the organisms and enzymes involved. We rationalized that simple enzymic synthesis of inositol hexakisphosphate labeled with 32P would
greatly enable study of transformation of soil inositol phosphates when combined with robust HPLC separations of different inositol phosphates.
Original languageEnglish
Pages (from-to)149–161
JournalPlant and Soil
Volume427
Issue number1-2
Early online date27 Jun 2017
DOIs
Publication statusPublished - Jun 2018

Keywords

  • Phytate
  • neo-
  • 1D-chiro-
  • myo-
  • scyllo-inositol hexakisphosphate
  • Phosphate

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