Single-cell microfluidic platform to study anaerobic bacteria

Yanqing Song, Andrew Glidle, Christopher Quince, Gavin Collins, William Sloan, Huabing Yin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel, anaerobic microfluidic system, for real-time monitoring of strictly anaerobic bacteria with single-cell resolution. Chemotactic responses of a cellulolytic bacterium Clostridium Cellulolyticum towards cellobiose were characterized using a gradient microfluidic device. The degradation of microcrystalline cellulose by C. cellulolyticum was observed in parallel microfluidic channels, suggesting that chemotaxis may play a key role in cellulose degradation by anaerobic digesters. These results demonstrate that microfluidics has great potential to study anaerobic microbial communities.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages508-509
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 27 Oct 201931 Oct 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period27/10/1931/10/19

Keywords

  • Anaerobic bacteria
  • Cellulose
  • Chemotaxis
  • Microfluidics
  • Single-cell assay

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