Smad1 transcription factor integrates BMP2 and Wnt3a signals in migrating cardiac progenitor cells

Junfang Song, James Mccoll, Ester Camp Navarro, Nicola Kennerley, Geoffrey Mok, Dominique Mccormick, Timothy Grocott, Grant Wheeler, Andrea Munsterberg

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)
12 Downloads (Pure)

Abstract

In vertebrate embryos, cardiac progenitor cells (CPCs) undergo long-range migration after emerging from the primitive streak during gastrulation. Together with other mesoderm progenitors, they migrate laterally and then toward the ventral midline, where they form the heart. Signals controlling the migration of different progenitor cell populations during gastrulation are poorly understood. Several pathways are involved in the epithelial-to-mesenchymal transition and ingression of mesoderm cells through the primitive streak, including fibroblast growth factors and wingless-type family members (Wnt). Here we focus on early CPC migration and use live video microscopy in chicken embryos to demonstrate a role for bone morphogenetic protein (BMP)/SMA and MAD related (Smad) signaling. We identify an interaction of BMP and Wnt/glycogen synthase kinase 3 beta (GSK3β) pathways via the differential phosphorylation of Smad1. Increased BMP2 activity altered migration trajectories of prospective cardiac cells and resulted in their lateral displacement and ectopic differentiation, as they failed to reach the ventral midline. Constitutively active BMP receptors or constitutively active Smad1 mimicked this phenotype, suggesting a cell autonomous response. Expression of GSK3β, which promotes the turnover of active Smad1, rescued the BMP-induced migration phenotype. Conversely, expression of GSK3β-resistant Smad1 resulted in aberrant CPC migration trajectories. De-repression of GSK3β by dominant negative Wnt3a restored normal migration patterns in the presence of high BMP activity. The data indicate the convergence of BMP and Wnt pathways on Smad1 during the early migration of prospective cardiac cells. Overall, we reveal molecular mechanisms that contribute to the emerging paradigm of signaling pathway integration in embryo development.
Original languageEnglish
Pages (from-to)7337-7342
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume111
Issue number20
Early online date12 May 2014
DOIs
Publication statusPublished - 20 May 2014

Keywords

  • live imaging
  • cell tracking

Cite this