Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters

Ping Xu, Irina Mohorianu, Li Yang, Hansheng Zhao, Zhimin Gao, Tamas Dalmay

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Abstract

Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed. 

Original languageEnglish
Article numbere103590
JournalPLoS One
Volume9
Issue number7
DOIs
Publication statusPublished - 31 Jul 2014

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