Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters

Ping Xu; Irina Mohorianu; Li Yang; Hansheng Zhao; Zhimin Gao; Tamas Dalmay

doi:10.1371/journal.pone.0103590

Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters

Ping Xu, Irina Mohorianu, Li Yang, Hansheng Zhao, Zhimin Gao, Tamas Dalmay

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

17 Downloads (Pure)

Abstract

Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed.

Original language	English
Article number	e103590
Journal	PLoS One
Volume	9
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0103590
Publication status	Published - 31 Jul 2014

Access to Document

10.1371/journal.pone.0103590Licence: CC BY

Xu_etal_2014_PLOSONE
© 2014 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Final published version, 392 KBLicence: CC BY

Tamas Dalmay
- T.Dalmayuea.acuk
- School of Biological Sciences - Professor of RNA Biology
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching & Research

Developing molecular biology kits for more sensitive diagnostics and gene expression analysis
Dalmay, T., Gray, J. & Sorefan, K.
Biotechnology and Biological Sciences Research Council
30/08/12 → 29/08/13
Project: Research

Cite this

@article{55fb5b2c5fa54c3e907e9fa58f4e9dc6,

title = "Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters",

abstract = "Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed. ",

author = "Ping Xu and Irina Mohorianu and Li Yang and Hansheng Zhao and Zhimin Gao and Tamas Dalmay",

note = "{\textcopyright} 2014 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2014",

month = jul,

day = "31",

doi = "10.1371/journal.pone.0103590",

language = "English",

volume = "9",

journal = "PLoS One",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "7",

}

TY - JOUR

T1 - Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters

AU - Xu, Ping

AU - Mohorianu, Irina

AU - Yang, Li

AU - Zhao, Hansheng

AU - Gao, Zhimin

AU - Dalmay, Tamas

N1 - © 2014 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2014/7/31

Y1 - 2014/7/31

N2 - Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed.

AB - Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed.

U2 - 10.1371/journal.pone.0103590

DO - 10.1371/journal.pone.0103590

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 7

M1 - e103590

ER -

Small RNA profile in Moso bamboo root and leaf obtained by high definition adapters

Abstract

Access to Document

Profiles

Tamas Dalmay

Projects

Developing molecular biology kits for more sensitive diagnostics and gene expression analysis

Cite this