Solanum tuberosum inositol phosphate kinase (StlTPK1) displaying inositol phosphate-inositol phosphate and inositol phosphate-ADP phosphotransferase activities

Samuel E. K. Caddick; Christopher J. Harrison; Ioanna Stavridou; Jennifer L. Mitchell; Andrew M. Hemmings; Charles A. Brearley

doi:10.1016/j.febslet.2008.04.034

Solanum tuberosum inositol phosphate kinase (StlTPK1) displaying inositol phosphate-inositol phosphate and inositol phosphate-ADP phosphotransferase activities

Samuel E. K. Caddick, Christopher J. Harrison, Ioanna Stavridou, Jennifer L. Mitchell, Andrew M. Hemmings, Charles A. Brearley

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We describe a multifunctional inositol polyphosphate kinase/phosphotransferase from Solanum tuberosum, StITPKa (GenBank accession number: EF362784), hereafter called StITPK1. StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity: Km = 27 µM, and Vmax = 19 nmol min-1 mg-1. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate–ADP phosphotransferase activity in the presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. Homology modelling provides a structural rationale of the catalytic abilities of StITPK1. Inter-substrate transfer of phosphate groups between inositol phosphates is an evolutionarily conserved function of enzymes of this class.

Original language	English
Pages (from-to)	1731-1737
Number of pages	7
Journal	FEBS Letters
Volume	582
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2008.04.034
Publication status	Published - 28 May 2008

Access to Document

10.1016/j.febslet.2008.04.034

Cite this

@article{bf4ad97ae68d4e16850613434b7a16fb,

title = "Solanum tuberosum inositol phosphate kinase (StlTPK1) displaying inositol phosphate-inositol phosphate and inositol phosphate-ADP phosphotransferase activities",

abstract = "We describe a multifunctional inositol polyphosphate kinase/phosphotransferase from Solanum tuberosum, StITPKa (GenBank accession number: EF362784), hereafter called StITPK1. StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity: Km = 27 µM, and Vmax = 19 nmol min-1 mg-1. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate–ADP phosphotransferase activity in the presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. Homology modelling provides a structural rationale of the catalytic abilities of StITPK1. Inter-substrate transfer of phosphate groups between inositol phosphates is an evolutionarily conserved function of enzymes of this class.",

author = "Caddick, {Samuel E. K.} and Harrison, {Christopher J.} and Ioanna Stavridou and Mitchell, {Jennifer L.} and Hemmings, {Andrew M.} and Brearley, {Charles A.}",

year = "2008",

month = may,

day = "28",

doi = "10.1016/j.febslet.2008.04.034",

language = "English",

volume = "582",

pages = "1731--1737",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "12",

}

TY - JOUR

T1 - Solanum tuberosum inositol phosphate kinase (StlTPK1) displaying inositol phosphate-inositol phosphate and inositol phosphate-ADP phosphotransferase activities

AU - Caddick, Samuel E. K.

AU - Harrison, Christopher J.

AU - Stavridou, Ioanna

AU - Mitchell, Jennifer L.

AU - Hemmings, Andrew M.

AU - Brearley, Charles A.

PY - 2008/5/28

Y1 - 2008/5/28

N2 - We describe a multifunctional inositol polyphosphate kinase/phosphotransferase from Solanum tuberosum, StITPKa (GenBank accession number: EF362784), hereafter called StITPK1. StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity: Km = 27 µM, and Vmax = 19 nmol min-1 mg-1. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate–ADP phosphotransferase activity in the presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. Homology modelling provides a structural rationale of the catalytic abilities of StITPK1. Inter-substrate transfer of phosphate groups between inositol phosphates is an evolutionarily conserved function of enzymes of this class.

AB - We describe a multifunctional inositol polyphosphate kinase/phosphotransferase from Solanum tuberosum, StITPKa (GenBank accession number: EF362784), hereafter called StITPK1. StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity: Km = 27 µM, and Vmax = 19 nmol min-1 mg-1. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate–ADP phosphotransferase activity in the presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. Homology modelling provides a structural rationale of the catalytic abilities of StITPK1. Inter-substrate transfer of phosphate groups between inositol phosphates is an evolutionarily conserved function of enzymes of this class.

U2 - 10.1016/j.febslet.2008.04.034

DO - 10.1016/j.febslet.2008.04.034

M3 - Article

VL - 582

SP - 1731

EP - 1737

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 12

ER -