Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates

Gerard Giraud; Holger Schulze; Till T. Bachmann; Colin J. Campbell; Andrew R. Mount; Peter Ghazal; Mizanur R. Khondoker; Stuart W.J. Ember; Ilenia Ciani; Chaker Tlili; Anthony J. Walton; Jonathan G. Terry; Jason Crain

doi:10.1016/j.cplett.2009.11.032

Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates

Gerard Giraud, Holger Schulze, Till T. Bachmann, Colin J. Campbell, Andrew R. Mount, Peter Ghazal, Mizanur R. Khondoker, Stuart W.J. Ember, Ilenia Ciani, Chaker Tlili, Anthony J. Walton, Jonathan G. Terry, Jason Crain

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe)ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD–DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 μs (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots.

Original language	English
Pages (from-to)	309-314
Number of pages	6
Journal	Chemical Physics Letters
Volume	484
Issue number	4-6
Early online date	18 Nov 2009
DOIs	https://doi.org/10.1016/j.cplett.2009.11.032
Publication status	Published - 7 Jan 2010

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10.1016/j.cplett.2009.11.032

http://www.sciencedirect.com/science/article/pii/S0009261409014560

Cite this

Giraud, G., Schulze, H., Bachmann, T. T., Campbell, C. J., Mount, A. R., Ghazal, P., Khondoker, M. R., Ember, S. W. J., Ciani, I., Tlili, C., Walton, A. J., Terry, J. G., & Crain, J. (2010). Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates. Chemical Physics Letters, 484(4-6), 309-314. https://doi.org/10.1016/j.cplett.2009.11.032

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title = "Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates",

abstract = "In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe)ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD–DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 μs (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots.",

author = "Gerard Giraud and Holger Schulze and Bachmann, {Till T.} and Campbell, {Colin J.} and Mount, {Andrew R.} and Peter Ghazal and Khondoker, {Mizanur R.} and Ember, {Stuart W.J.} and Ilenia Ciani and Chaker Tlili and Walton, {Anthony J.} and Terry, {Jonathan G.} and Jason Crain",

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Giraud, G, Schulze, H, Bachmann, TT, Campbell, CJ, Mount, AR, Ghazal, P, Khondoker, MR, Ember, SWJ, Ciani, I, Tlili, C, Walton, AJ, Terry, JG & Crain, J 2010, 'Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates', Chemical Physics Letters, vol. 484, no. 4-6, pp. 309-314. https://doi.org/10.1016/j.cplett.2009.11.032

TY - JOUR

T1 - Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates

AU - Giraud, Gerard

AU - Schulze, Holger

AU - Bachmann, Till T.

AU - Campbell, Colin J.

AU - Mount, Andrew R.

AU - Ghazal, Peter

AU - Khondoker, Mizanur R.

AU - Ember, Stuart W.J.

AU - Ciani, Ilenia

AU - Tlili, Chaker

AU - Walton, Anthony J.

AU - Terry, Jonathan G.

AU - Crain, Jason

PY - 2010/1/7

Y1 - 2010/1/7

N2 - In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe)ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD–DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 μs (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots.

AB - In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe)ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD–DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 μs (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots.

U2 - 10.1016/j.cplett.2009.11.032

DO - 10.1016/j.cplett.2009.11.032

M3 - Article

VL - 484

SP - 309

EP - 314

JO - Chemical Physics Letters

JF - Chemical Physics Letters

SN - 0009-2614

IS - 4-6

ER -