Specific ER quality control components required for biogenesis of the plant innate immune receptor EFR

Jing Li, Chu Zhao-Hui, Martine Batoux, Vladimir Nekrasov, Milena Roux, Delphine Chinchilla, Cyril Zipfel, Jonathan D G Jones

Research output: Contribution to journalArticlepeer-review

187 Citations (Scopus)

Abstract

Plant innate immunity depends in part on recognition of pathogen-associated molecular patterns (PAMPs), such as bacterial flagellin, EF-Tu, and fungal chitin. Recognition is mediated by pattern-recognition receptors (PRRs) and results in PAMP-triggered immunity. EF-Tu and flagellin, and the derived peptides elf18 and flg22, are recognized in Arabidopsis by the leucine-rich repeat receptor kinases (LRR-RK), EFR and FLS2, respectively. To gain insights into the molecular mechanisms underlying PTI, we investigated EFR-mediated PTI using genetics. A forward-genetic screen for Arabidopsis elf18-insensitive (elfin) mutants revealed multiple alleles of calreticulin3 (CRT3), UDP-glucose glycoprotein glucosyl transferase (UGGT), and an HDEL receptor family member (ERD2b), potentially involved in endoplasmic reticulum quality control (ER-QC). Strikingly, FLS2-mediated responses were not impaired in crt3, uggt, and erd2b null mutants, revealing that the identified mutations are specific to EFR. A crt3 null mutant did not accumulate EFR protein, suggesting that EFR is a substrate for CRT3. Interestingly, Erd2b did not accumulate CRT3 protein, although they accumulate wild-type levels of other ER proteins. ERD2B seems therefore to be a specific HDEL receptor for CRT3 that allows its retro-translocation from the Golgi to the ER. These data reveal a previously unsuspected role of a specific subset of ER-QC machinery components for PRR accumulation in plant innate immunity.
Original languageEnglish
Pages (from-to)15973-15978
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America (PNAS)
Volume106
Issue number37
DOIs
Publication statusPublished - 15 Sep 2009

Keywords

  • Alleles
  • Arabidopsis
  • Arabidopsis Proteins
  • Base Sequence
  • Calreticulin
  • DNA Primers
  • Endoplasmic Reticulum
  • Genes, Plant
  • Glucosyltransferases
  • Host-Pathogen Interactions
  • Immunity, Innate
  • Membrane Proteins
  • Mutation
  • Plant Diseases
  • Plants, Genetically Modified
  • Protein Kinases
  • Pseudomonas syringae
  • Signal Transduction

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