Spin Saturation Transfer Difference NMR (SSTD NMR): a new tool to obtain kinetic parameters of chemical exchange processes

María Teresa Quirós, Colin Macdonald, Jesús Angulo, María Paz

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This detailed protocol describes the new Spin Saturation Transfer Difference Nuclear Magnetic Resonance protocol (SSTD NMR), recently developed in our group to study processes of mutual-site chemical exchange that are difficult to analyze by traditional methods. As the name suggests, this method combines the Spin Saturation Transfer method used for small molecules, with the Saturation Transfer Difference (STD) NMR method employed for the study of protein-ligand interactions, by measuring transient spin saturation transfer along increasing saturation times (build-up curves) in small organic and organometallic molecules undergoing chemical exchange.
Original languageEnglish
Article numbere54499
JournalJournal of visualized experiments : JoVE
Issue number117
Publication statusPublished - 12 Nov 2016


  • NMMR
  • spin saturation transfer
  • saturation transfer difference
  • kinetics
  • chemical exchange
  • NOE
  • thermodynamic parameters

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