Projects per year
Abstract
Background: Negative regulators of signal transduction cascades play critical roles in controlling different aspects of normal embryonic development. Sprouty2 (Spry2) negatively regulates receptor tyrosine kinases (RTK) and FGF signalling and is important in differentiation, cell migration and proliferation. In vertebrate embryos, Spry2 is expressed in paraxial mesoderm and in forming somites. Expression is maintained in the myotome until late stages of somite differentiation. However, its role and mode of action during somite myogenesis is still unclear.
Results: Here, we analysed chick Spry2 expression and showed that it overlaps with that of myogenic regulatory factors MyoD and Mgn. Targeted mis-expression of Spry2 led to inhibition of myogenesis, whilst its C-terminal domain led to an increased number of myogenic cells by stimulating cell proliferation.
Conclusions: Spry2 is expressed in somite myotomes and its expression overlaps with myogenic regulatory factors.
Overexpression and dominant-negative interference showed that Spry2 plays a crucial role in regulating chick myogenesis by fine tuning of FGF signaling through a negative feedback loop. We also propose that mir-23, mir-27 and mir-128 could be part of the negative feedback loop mechanism. Our analysis is the first to shed some light on in vivo Spry2 function during chick somite myogenesis.
Results: Here, we analysed chick Spry2 expression and showed that it overlaps with that of myogenic regulatory factors MyoD and Mgn. Targeted mis-expression of Spry2 led to inhibition of myogenesis, whilst its C-terminal domain led to an increased number of myogenic cells by stimulating cell proliferation.
Conclusions: Spry2 is expressed in somite myotomes and its expression overlaps with myogenic regulatory factors.
Overexpression and dominant-negative interference showed that Spry2 plays a crucial role in regulating chick myogenesis by fine tuning of FGF signaling through a negative feedback loop. We also propose that mir-23, mir-27 and mir-128 could be part of the negative feedback loop mechanism. Our analysis is the first to shed some light on in vivo Spry2 function during chick somite myogenesis.
Original language | English |
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Article number | S8 |
Journal | BMC Medical Genomics |
Volume | 8 |
Issue number | Suppl 1 |
DOIs | |
Publication status | Published - 15 Jan 2015 |
Profiles
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Andrea Münsterberg
- School of Biological Sciences - Professor of Developmental Biology
- Cells and Tissues - Member
Person: Research Group Member, Academic, Teaching & Research
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Grant Wheeler
- School of Biological Sciences - Professor
- Cells and Tissues - Member
- Wheeler Group - Group Leader
Person: Group Lead, Research Group Member, Academic, Teaching & Research
Projects
- 1 Finished
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Investigating FGK ERK MAP kinase signaling in vertebrate skeletal muscle differentiation
1/06/07 → 31/08/10
Project: Research