Stable isotope probing - linking microbial identity to function

Marc G Dumont; J Colin Murrell

doi:10.1038/nrmicro1162

Stable isotope probing - linking microbial identity to function

Marc G Dumont, J Colin Murrell

Research output: Contribution to journal › Article › peer-review

464 Citations (Scopus)

Abstract

Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as (13)C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and (13)C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.

Original language	English
Pages (from-to)	499-504
Number of pages	6
Journal	Nature Reviews Microbiology
Volume	3
Issue number	6
DOIs	https://doi.org/10.1038/nrmicro1162
Publication status	Published - Jun 2005

Keywords

Bacteria
Carbon Isotopes
DNA, Bacterial
Isotope Labeling
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S

Access to Document

10.1038/nrmicro1162

Cite this

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KW - Bacteria

KW - Carbon Isotopes

KW - DNA, Bacterial

KW - Isotope Labeling

KW - Phylogeny

KW - Polymerase Chain Reaction

KW - RNA, Ribosomal, 16S

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