Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Nicola J. Patron; Diego Orzaez; Sylvestre Marillonnet; Heribert Warzecha; Colette Matthewman; Mark Youles; Oleg Raitskin; Aymeric Leveau; Gemma Farré; Christian Rogers; Alison Smith; Julian Hibberd; Alex A. R. Webb; James Locke; Sebastian Schornack; Jim Ajioka; David C. Baulcombe; Cyril Zipfel; Sophien Kamoun; Jonathan D. G. Jones; Hannah Kuhn; Silke Robatzek; H. Peter Van Esse; Dale Sanders; Giles Oldroyd; Cathie Martin; Rob Field; Sarah O'Connor; Samantha Fox; Brande Wulff; Ben Miller; Andy Breakspear; Guru Radhakrishnan; Pierre-Marc Delaux; Dominique Loqué; Antonio Granell; Alain Tissier; Patrick Shih; Thomas P. Brutnell; W. Paul Quick; Heiko Rischer; Paul D. Fraser; Asaph Aharoni; Christine Raines; Paul F. South; Jean-Michel Ané; Björn R. Hamberger; Jane Langdale; Jens Stougaard; Harro Bouwmeester; Michael Udvardi; James A. H. Murray; Vardis Ntoukakis; Patrick Schäfer; Katherine Denby; Keith J. Edwards; Anne Osbourn; Jim Haseloff

doi:10.1111/nph.13532

Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Nicola J. Patron, Diego Orzaez, Sylvestre Marillonnet, Heribert Warzecha, Colette Matthewman, Mark Youles, Oleg Raitskin, Aymeric Leveau, Gemma Farré, Christian Rogers, Alison Smith, Julian Hibberd, Alex A. R. Webb, James Locke, Sebastian Schornack, Jim Ajioka, David C. Baulcombe, Cyril Zipfel, Sophien Kamoun, Jonathan D. G. JonesHannah Kuhn, Silke Robatzek, H. Peter Van Esse, Dale Sanders, Giles Oldroyd, Cathie Martin, Rob Field, Sarah O'Connor, Samantha Fox, Brande Wulff, Ben Miller, Andy Breakspear, Guru Radhakrishnan, Pierre-Marc Delaux, Dominique Loqué, Antonio Granell, Alain Tissier, Patrick Shih, Thomas P. Brutnell, W. Paul Quick, Heiko Rischer, Paul D. Fraser, Asaph Aharoni, Christine Raines, Paul F. South, Jean-Michel Ané, Björn R. Hamberger, Jane Langdale, Jens Stougaard, Harro Bouwmeester, Michael Udvardi, James A. H. Murray, Vardis Ntoukakis, Patrick Schäfer, Katherine Denby, Keith J. Edwards, Anne Osbourn, Jim Haseloff

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Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Original language	English
Pages (from-to)	13-19
Number of pages	7
Journal	New Phytologist
Volume	208
Issue number	1
Early online date	14 Jul 2015
DOIs	https://doi.org/10.1111/nph.13532
Publication status	Published - Oct 2015

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10.1111/nph.13532

Standards for Plant Synthetic Biology (Miller) FINALAccepted author manuscript, 241 KB

Jonathan Jones
- Jonathan.Jonesuea.acuk
- School of Biological Sciences - Professor of Biology
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching & Research
Sophien Kamoun
- S.Kamounuea.acuk
- School of Biological Sciences - Professor of Biology
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching & Research
Ben Miller
- B.Milleruea.acuk
- School of Biological Sciences - Associate Professor
- Molecular Microbiology - Member
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching & Research

OpenPlant
Patron, N. & Jones, J.
Biotechnology and Biological Sciences Research Council
31/07/14 → 31/03/22
Project: Research

Cite this

Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., Raitskin, O., Leveau, A., Farré, G., Rogers, C., Smith, A., Hibberd, J., Webb, A. A. R., Locke, J., Schornack, S., Ajioka, J., Baulcombe, D. C., Zipfel, C., Kamoun, S., ... Haseloff, J. (2015). Standards for plant synthetic biology: A common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. https://doi.org/10.1111/nph.13532

@article{1996e3958b0740dd8f386e3e5075d646,

title = "Standards for plant synthetic biology: A common syntax for exchange of DNA parts",

abstract = "Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.",

author = "Patron, {Nicola J.} and Diego Orzaez and Sylvestre Marillonnet and Heribert Warzecha and Colette Matthewman and Mark Youles and Oleg Raitskin and Aymeric Leveau and Gemma Farr{\'e} and Christian Rogers and Alison Smith and Julian Hibberd and Webb, {Alex A. R.} and James Locke and Sebastian Schornack and Jim Ajioka and Baulcombe, {David C.} and Cyril Zipfel and Sophien Kamoun and Jones, {Jonathan D. G.} and Hannah Kuhn and Silke Robatzek and {Van Esse}, {H. Peter} and Dale Sanders and Giles Oldroyd and Cathie Martin and Rob Field and Sarah O'Connor and Samantha Fox and Brande Wulff and Ben Miller and Andy Breakspear and Guru Radhakrishnan and Pierre-Marc Delaux and Dominique Loqu{\'e} and Antonio Granell and Alain Tissier and Patrick Shih and Brutnell, {Thomas P.} and Quick, {W. Paul} and Heiko Rischer and Fraser, {Paul D.} and Asaph Aharoni and Christine Raines and South, {Paul F.} and Jean-Michel An{\'e} and Hamberger, {Bj{\"o}rn R.} and Jane Langdale and Jens Stougaard and Harro Bouwmeester and Michael Udvardi and Murray, {James A. H.} and Vardis Ntoukakis and Patrick Sch{\"a}fer and Katherine Denby and Edwards, {Keith J.} and Anne Osbourn and Jim Haseloff",

note = "{\textcopyright} 2015 The Authors. New Phytologist {\textcopyright} 2015 New Phytologist Trust.",

year = "2015",

month = oct,

doi = "10.1111/nph.13532",

language = "English",

volume = "208",

pages = "13--19",

journal = "New Phytologist",

issn = "0028-646X",

publisher = "Wiley",

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}

Patron, NJ, Orzaez, D, Marillonnet, S, Warzecha, H, Matthewman, C , Youles, M, Raitskin, O, Leveau, A, Farré, G, Rogers, C, Smith, A, Hibberd, J, Webb, AAR, Locke, J, Schornack, S, Ajioka, J, Baulcombe, DC, Zipfel, C , Kamoun, S , Jones, JDG, Kuhn, H, Robatzek, S, Van Esse, HP, Sanders, D, Oldroyd, G, Martin, C, Field, R, O'Connor, S, Fox, S, Wulff, B, Miller, B, Breakspear, A, Radhakrishnan, G, Delaux, P-M, Loqué, D, Granell, A, Tissier, A, Shih, P, Brutnell, TP, Quick, WP, Rischer, H, Fraser, PD, Aharoni, A, Raines, C, South, PF, Ané, J-M, Hamberger, BR, Langdale, J, Stougaard, J, Bouwmeester, H, Udvardi, M, Murray, JAH, Ntoukakis, V, Schäfer, P, Denby, K, Edwards, KJ, Osbourn, A & Haseloff, J 2015, 'Standards for plant synthetic biology: A common syntax for exchange of DNA parts', New Phytologist, vol. 208, no. 1, pp. 13-19. https://doi.org/10.1111/nph.13532

TY - JOUR

T1 - Standards for plant synthetic biology: A common syntax for exchange of DNA parts

AU - Patron, Nicola J.

AU - Orzaez, Diego

AU - Marillonnet, Sylvestre

AU - Warzecha, Heribert

AU - Matthewman, Colette

AU - Youles, Mark

AU - Raitskin, Oleg

AU - Leveau, Aymeric

AU - Farré, Gemma

AU - Rogers, Christian

AU - Smith, Alison

AU - Hibberd, Julian

AU - Webb, Alex A. R.

AU - Locke, James

AU - Schornack, Sebastian

AU - Ajioka, Jim

AU - Baulcombe, David C.

AU - Zipfel, Cyril

AU - Kamoun, Sophien

AU - Jones, Jonathan D. G.

AU - Kuhn, Hannah

AU - Robatzek, Silke

AU - Van Esse, H. Peter

AU - Sanders, Dale

AU - Oldroyd, Giles

AU - Martin, Cathie

AU - Field, Rob

AU - O'Connor, Sarah

AU - Fox, Samantha

AU - Wulff, Brande

AU - Miller, Ben

AU - Breakspear, Andy

AU - Radhakrishnan, Guru

AU - Delaux, Pierre-Marc

AU - Loqué, Dominique

AU - Granell, Antonio

AU - Tissier, Alain

AU - Shih, Patrick

AU - Brutnell, Thomas P.

AU - Quick, W. Paul

AU - Rischer, Heiko

AU - Fraser, Paul D.

AU - Aharoni, Asaph

AU - Raines, Christine

AU - South, Paul F.

AU - Ané, Jean-Michel

AU - Hamberger, Björn R.

AU - Langdale, Jane

AU - Stougaard, Jens

AU - Bouwmeester, Harro

AU - Udvardi, Michael

AU - Murray, James A. H.

AU - Ntoukakis, Vardis

AU - Schäfer, Patrick

AU - Denby, Katherine

AU - Edwards, Keith J.

AU - Osbourn, Anne

AU - Haseloff, Jim

PY - 2015/10

Y1 - 2015/10

N2 - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

AB - Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

U2 - 10.1111/nph.13532

DO - 10.1111/nph.13532

M3 - Article

C2 - 26171760

VL - 208

SP - 13

EP - 19

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -

Standards for plant synthetic biology: A common syntax for exchange of DNA parts

Abstract

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Jonathan Jones

Sophien Kamoun

Ben Miller

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OpenPlant

Cite this