TY - JOUR
T1 - Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
AU - Hiebert, Colin W.
AU - Moscou, Matthew J.
AU - Hewitt, Tim
AU - Steuernagel, Burkhard
AU - Hernandez-Pinzon Toscano, Inmaculada
AU - Green, Phon
AU - Pujol, Vincent
AU - Zhang, Peng
AU - Rouse, Matthew N.
AU - Jin, Yue
AU - McIntosh, Robert A.
AU - Upadhyaya, Narayana
AU - Zhang, Jianping
AU - Bhavani, Sridhar
AU - Vrána, Jan
AU - Karafiátová, Miroslava
AU - Huang, Li
AU - Fetch, Tom
AU - Doležel, Jaroslav
AU - Wulff, Brande B. H.
AU - Lagudah, Evans
AU - Spielmeyer, Wolfgang
PY - 2020/2/28
Y1 - 2020/2/28
N2 - Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat. Stem rust is an important disease of wheat and resistance present in some cultivars can be suppressed by the SuSr-D1 locus. Here the authors show that SuSr-D1 encodes a subunit of the Mediator Complex and that nonsense mutations are sufficient to abolish suppression and confer stem rust resistance.
AB - Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat. Stem rust is an important disease of wheat and resistance present in some cultivars can be suppressed by the SuSr-D1 locus. Here the authors show that SuSr-D1 encodes a subunit of the Mediator Complex and that nonsense mutations are sufficient to abolish suppression and confer stem rust resistance.
KW - F-SP TRITICI
KW - AEGILOPS-TAUSCHII COSS.
KW - DEFENSE GENE-EXPRESSION
KW - LEAF RUST
KW - HEXAPLOID WHEAT
KW - COMMON WHEAT
KW - PHYLOGENETIC ANALYSIS
KW - STRIPE RUST
KW - AESTIVUM L
KW - RACE TTKS
UR - http://www.scopus.com/inward/record.url?scp=85080992910&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-14937-2
DO - 10.1038/s41467-020-14937-2
M3 - Article
C2 - 32111840
VL - 11
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1123
ER -