Stress-induced ATP release from and growth modulation of human lens and retinal pigment epithelial cells

J.A. Eldred; J. Sanderson; I.M. Wormstone; J.R. Reddan; G. Duncan

doi:10.1042/bst0311213

Stress-induced ATP release from and growth modulation of human lens and retinal pigment epithelial cells

J.A. Eldred, J. Sanderson, I.M. Wormstone, J.R. Reddan, G. Duncan

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

ATP release has been shown to occur following stimulation in several cellular systems. This study was undertaken to determine if lens and retinal epithelial cells release ATP in response to physiological stresses and to elucidate a possible role for ATP. Analysis of human aqueous humour samples showed a mean ATP level of 37.8+/-7.7 nM. Hyper-osmotic stress induced a dose- and time-dependent release of ATP. Both cell types were found to proliferate in serum-free medium, and the addition of ATP and adenosine at concentrations as low as 0.1 nM inhibited growth. Gene profiling also demonstrated the presence of the ectonucleotidases CD39 and CD73 and the A1 adenosine receptor on both cell types.

Original language	English
Pages (from-to)	1213-1215
Number of pages	3
Journal	Biochemical Society Transactions
Volume	31
Issue number	6
DOIs	https://doi.org/10.1042/bst0311213
Publication status	Published - 2003

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10.1042/bst0311213

Cite this

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title = "Stress-induced ATP release from and growth modulation of human lens and retinal pigment epithelial cells",

abstract = "ATP release has been shown to occur following stimulation in several cellular systems. This study was undertaken to determine if lens and retinal epithelial cells release ATP in response to physiological stresses and to elucidate a possible role for ATP. Analysis of human aqueous humour samples showed a mean ATP level of 37.8+/-7.7 nM. Hyper-osmotic stress induced a dose- and time-dependent release of ATP. Both cell types were found to proliferate in serum-free medium, and the addition of ATP and adenosine at concentrations as low as 0.1 nM inhibited growth. Gene profiling also demonstrated the presence of the ectonucleotidases CD39 and CD73 and the A1 adenosine receptor on both cell types.",

author = "J.A. Eldred and J. Sanderson and I.M. Wormstone and J.R. Reddan and G. Duncan",

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T1 - Stress-induced ATP release from and growth modulation of human lens and retinal pigment epithelial cells

AU - Eldred, J.A.

AU - Sanderson, J.

AU - Wormstone, I.M.

AU - Reddan, J.R.

AU - Duncan, G.

PY - 2003

Y1 - 2003

N2 - ATP release has been shown to occur following stimulation in several cellular systems. This study was undertaken to determine if lens and retinal epithelial cells release ATP in response to physiological stresses and to elucidate a possible role for ATP. Analysis of human aqueous humour samples showed a mean ATP level of 37.8+/-7.7 nM. Hyper-osmotic stress induced a dose- and time-dependent release of ATP. Both cell types were found to proliferate in serum-free medium, and the addition of ATP and adenosine at concentrations as low as 0.1 nM inhibited growth. Gene profiling also demonstrated the presence of the ectonucleotidases CD39 and CD73 and the A1 adenosine receptor on both cell types.

AB - ATP release has been shown to occur following stimulation in several cellular systems. This study was undertaken to determine if lens and retinal epithelial cells release ATP in response to physiological stresses and to elucidate a possible role for ATP. Analysis of human aqueous humour samples showed a mean ATP level of 37.8+/-7.7 nM. Hyper-osmotic stress induced a dose- and time-dependent release of ATP. Both cell types were found to proliferate in serum-free medium, and the addition of ATP and adenosine at concentrations as low as 0.1 nM inhibited growth. Gene profiling also demonstrated the presence of the ectonucleotidases CD39 and CD73 and the A1 adenosine receptor on both cell types.

U2 - 10.1042/bst0311213

DO - 10.1042/bst0311213

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JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

IS - 6

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