Structural insights into higher order assembly and function of the bacterial micro compartment protein PduA

Allan Pang, Stefanie Frank, Ian R. Brown, Martin J. Warren, Richard W. Pickersgill

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Background: PduA is a major shell protein in 1,2-propanediol utilization micro compartments. Results: The profound influence of Lys-26 and Arg-79 on hexagonal tiling is revealed both in the crystal and in vivo. Conclusion: Interactions seen in the crystal are important for sheet and nanotube assembly in the bacterial cytoplasm. Significance: This work provides knowledge for the engineering of PduA for stability and porosity for synthetic biology applications.

Original languageEnglish
Pages (from-to)22377-22384
Number of pages8
JournalJournal of Biological Chemistry
Volume289
Issue number32
DOIs
Publication statusPublished - 8 Aug 2014

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