Structural studies of microcosm dental plaques grown under different nutritional conditions

Jonathan Pratten; Christopher S. Andrews; Duncan Q. M. Craig; Michael Wilson

doi:10.1016/S0378-1097(00)00287-1

Structural studies of microcosm dental plaques grown under different nutritional conditions

Jonathan Pratten, Christopher S. Andrews, Duncan Q. M. Craig, Michael Wilson

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

The aim of this study was to investigate the structure of intact oral biofilms using confocal laser scanning microscopy (CLSM). Mixed-species biofilms were grown on enamel discs in a constant depth film fermenter. The biofilms were fed with a mucin-containing artificial saliva with or without sucrose supplementation. Biofilms were examined using a Wild-Leitz CLSM, operating in reflected light mode. The microstructure of non-supplemented biofilms was revealed to be complex, with stacks of bacteria developing over time, separated by clear channels. Sucrose-supplemented biofilms appeared to colonise the substratum more rapidly. The results of this study have shown that using CLSM it is possible to examine the structure of oral biofilms grown under conditions similar to those which would exist in vivo. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	215-218
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	189
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(00)00287-1
Publication status	Published - 2000

Keywords

MICROBIAL BIOFILMS
biofilm
SUCROSE
microcosm dental plaque
confocal laser scanning microscopy
sucrose

Access to Document

10.1016/S0378-1097(00)00287-1

Cite this

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title = "Structural studies of microcosm dental plaques grown under different nutritional conditions",

abstract = "The aim of this study was to investigate the structure of intact oral biofilms using confocal laser scanning microscopy (CLSM). Mixed-species biofilms were grown on enamel discs in a constant depth film fermenter. The biofilms were fed with a mucin-containing artificial saliva with or without sucrose supplementation. Biofilms were examined using a Wild-Leitz CLSM, operating in reflected light mode. The microstructure of non-supplemented biofilms was revealed to be complex, with stacks of bacteria developing over time, separated by clear channels. Sucrose-supplemented biofilms appeared to colonise the substratum more rapidly. The results of this study have shown that using CLSM it is possible to examine the structure of oral biofilms grown under conditions similar to those which would exist in vivo. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",

keywords = "MICROBIAL BIOFILMS, biofilm, SUCROSE, microcosm dental plaque, confocal laser scanning microscopy, sucrose",

author = "Jonathan Pratten and Andrews, {Christopher S.} and Craig, {Duncan Q. M.} and Michael Wilson",

year = "2000",

doi = "10.1016/S0378-1097(00)00287-1",

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volume = "189",

pages = "215--218",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

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TY - JOUR

T1 - Structural studies of microcosm dental plaques grown under different nutritional conditions

AU - Pratten, Jonathan

AU - Andrews, Christopher S.

AU - Craig, Duncan Q. M.

AU - Wilson, Michael

PY - 2000

Y1 - 2000

N2 - The aim of this study was to investigate the structure of intact oral biofilms using confocal laser scanning microscopy (CLSM). Mixed-species biofilms were grown on enamel discs in a constant depth film fermenter. The biofilms were fed with a mucin-containing artificial saliva with or without sucrose supplementation. Biofilms were examined using a Wild-Leitz CLSM, operating in reflected light mode. The microstructure of non-supplemented biofilms was revealed to be complex, with stacks of bacteria developing over time, separated by clear channels. Sucrose-supplemented biofilms appeared to colonise the substratum more rapidly. The results of this study have shown that using CLSM it is possible to examine the structure of oral biofilms grown under conditions similar to those which would exist in vivo. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - The aim of this study was to investigate the structure of intact oral biofilms using confocal laser scanning microscopy (CLSM). Mixed-species biofilms were grown on enamel discs in a constant depth film fermenter. The biofilms were fed with a mucin-containing artificial saliva with or without sucrose supplementation. Biofilms were examined using a Wild-Leitz CLSM, operating in reflected light mode. The microstructure of non-supplemented biofilms was revealed to be complex, with stacks of bacteria developing over time, separated by clear channels. Sucrose-supplemented biofilms appeared to colonise the substratum more rapidly. The results of this study have shown that using CLSM it is possible to examine the structure of oral biofilms grown under conditions similar to those which would exist in vivo. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - MICROBIAL BIOFILMS

KW - biofilm

KW - SUCROSE

KW - microcosm dental plaque

KW - confocal laser scanning microscopy

KW - sucrose

U2 - 10.1016/S0378-1097(00)00287-1

DO - 10.1016/S0378-1097(00)00287-1

M3 - Article

VL - 189

SP - 215

EP - 218

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -