TY - JOUR
T1 - Studies on the exchange of valine-oxygen during the biosynthesis of δ-(L-α-Aminoadipoyl)-L-cysteinyl-D-valine
AU - Baldwin, Jack E.
AU - Adlington, Robert M.
AU - Bird, Juliette W.
AU - Field, Robert A.
AU - O'Callaghan, Niamh M.
AU - Schofield, Christopher J.
PY - 1992
Y1 - 1992
N2 - Incorporation of [4-2H6,18O2]-valine into δ-(L)-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), by intact cells of Cephalosporium acremonium, demonstrated the intracellular exchange of one and both valine oxygen atoms. Incubation of [18O2]-valine with the purified ACV synthetase from C. acremonium gave exclusive incorporation of a single 18O label into ACV, consistent witheffectively non-reversible formation of a covalent valinoyl-ACV synthetase intermediate under in vitro conditions.
AB - Incorporation of [4-2H6,18O2]-valine into δ-(L)-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), by intact cells of Cephalosporium acremonium, demonstrated the intracellular exchange of one and both valine oxygen atoms. Incubation of [18O2]-valine with the purified ACV synthetase from C. acremonium gave exclusive incorporation of a single 18O label into ACV, consistent witheffectively non-reversible formation of a covalent valinoyl-ACV synthetase intermediate under in vitro conditions.
KW - biosynthesis
KW - Cephalosporium acremonium
KW - penicillin.
KW - [O]-valine incorporation
KW - δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV)
UR - http://www.scopus.com/inward/record.url?scp=0026503708&partnerID=8YFLogxK
U2 - 10.1016/S0040-4020(01)88205-3
DO - 10.1016/S0040-4020(01)88205-3
M3 - Article
AN - SCOPUS:0026503708
VL - 48
SP - 1099
EP - 1108
JO - Tetrahedron
JF - Tetrahedron
SN - 0040-4020
IS - 6
ER -