TY - JOUR
T1 - Suppression of neutrophil superoxide generation by BNP is attenuated in acute heart failure: a case for 'BNP resistance'
AU - Liu, Saifei
AU - Ngo, Doan T. M.
AU - Chong, Cher-Rin
AU - Amarasekera, Anjalee T.
AU - Procter, Nathan E. K.
AU - Licari, Giovanni
AU - Dautov, Rustem F.
AU - Stewart, Simon
AU - Chirkov, Yuliy Y.
AU - Horowitz, John D.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Aims:
The release of the B‐type natriuretic peptide (BNP) is increased in heart failure (HF), a condition associated with oxidative stress. BNP is known to exert anti‐inflammatory effects including suppression of neutrophil superoxide (O2−) release. However, BNP‐based restoration of homeostasis in HF is inadequate, and the equivocal clinical benefit of a recombinant BNP, nesiritide, raises the possibility of attenuated response to BNP. We therefore tested the hypothesis that BNP‐induced suppression of neutrophil O2− generation is impaired in patients with acute HF.
Methods and results:
We have recently characterized suppression of neutrophil O2− generation (PMA‐ or fMLP‐stimulated neutrophil burst) by BNP as a measure of its physiological activity. In the present study, BNP response was compared in neutrophils of healthy subjects (n = 29) and HF patients (n = 45). Effects of BNP on fMLP‐induced phosphorylation of the NAD(P)H oxidase subunit p47phox were also evaluated. In acute HF patients, the suppressing effect of BNP (1 µmol/L) on O2− generation was attenuated relative to that in healthy subjects (P < 0.05 for both PMA and fMLP). Analogously, BNP inhibited p47phox phosphorylation in healthy subjects but not in HF patients (P < 0.05). However, O2−‐suppressing effects of the cell‐permeable cGMP analogue (8‐pCPT‐cGMP) were preserved in acute HF. Conventional HF treatment for 5 weeks partially restored neutrophil BNP responsiveness (n = 25, P < 0.05), despite no significant decrease in plasma NT‐proBNP levels.
Conclusions:
BNP inhibits neutrophil O2− generation by suppressing NAD(P)H oxidase assembly. This effect is impaired in acute HF patients, with partial recovery during treatment.
AB - Aims:
The release of the B‐type natriuretic peptide (BNP) is increased in heart failure (HF), a condition associated with oxidative stress. BNP is known to exert anti‐inflammatory effects including suppression of neutrophil superoxide (O2−) release. However, BNP‐based restoration of homeostasis in HF is inadequate, and the equivocal clinical benefit of a recombinant BNP, nesiritide, raises the possibility of attenuated response to BNP. We therefore tested the hypothesis that BNP‐induced suppression of neutrophil O2− generation is impaired in patients with acute HF.
Methods and results:
We have recently characterized suppression of neutrophil O2− generation (PMA‐ or fMLP‐stimulated neutrophil burst) by BNP as a measure of its physiological activity. In the present study, BNP response was compared in neutrophils of healthy subjects (n = 29) and HF patients (n = 45). Effects of BNP on fMLP‐induced phosphorylation of the NAD(P)H oxidase subunit p47phox were also evaluated. In acute HF patients, the suppressing effect of BNP (1 µmol/L) on O2− generation was attenuated relative to that in healthy subjects (P < 0.05 for both PMA and fMLP). Analogously, BNP inhibited p47phox phosphorylation in healthy subjects but not in HF patients (P < 0.05). However, O2−‐suppressing effects of the cell‐permeable cGMP analogue (8‐pCPT‐cGMP) were preserved in acute HF. Conventional HF treatment for 5 weeks partially restored neutrophil BNP responsiveness (n = 25, P < 0.05), despite no significant decrease in plasma NT‐proBNP levels.
Conclusions:
BNP inhibits neutrophil O2− generation by suppressing NAD(P)H oxidase assembly. This effect is impaired in acute HF patients, with partial recovery during treatment.
U2 - 10.1002/ejhf.242
DO - 10.1002/ejhf.242
M3 - Article
SN - 1388-9842
VL - 17
SP - 475
EP - 483
JO - European Journal of Heart Failure
JF - European Journal of Heart Failure
IS - 5
ER -