Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions

Grace Walden, Xin Liao, Graham Riley, Simon Donell, Michael J. Raxworthy, Aram Saeed

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Abstract

The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.
Original languageEnglish
Pages (from-to)531–535
JournalBioconjugate Chemistry
Volume30
Issue number3
Early online date7 Feb 2019
DOIs
Publication statusPublished - 20 Mar 2019

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