Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions

Grace Walden; Xin Liao; Graham Riley; Simon Donell; Michael J. Raxworthy; Aram Saeed

doi:10.1021/acs.bioconjchem.8b00868

Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions

Grace Walden
, Xin Liao
, Graham Riley
, Simon Donell
, Michael J. Raxworthy
, Aram Saeed

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

124 Downloads (Pure)

Abstract

The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.

Original language	English
Pages (from-to)	531–535
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	30
Issue number	3
Early online date	7 Feb 2019
DOIs	https://doi.org/10.1021/acs.bioconjchem.8b00868
Publication status	Published - 20 Mar 2019

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10.1021/acs.bioconjchem.8b00868

Bioconjugate Chemistry Communication_Grace Walden_accepted versionAccepted author manuscript, 1.46 MB
Supplementary Information Bioconjugate Chemistry Communication_Accepted versionOther version, 855 KB
TOC graphic accepted versionOther version, 186 KB

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Walden, G., Liao, X., Riley, G., Donell, S., Raxworthy, M. J., & Saeed, A. (2019). Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions. Bioconjugate Chemistry, 30(3), 531–535. https://doi.org/10.1021/acs.bioconjchem.8b00868

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abstract = "The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.",

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Walden, G, Liao, X, Riley, G, Donell, S, Raxworthy, MJ & Saeed, A 2019, 'Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions', Bioconjugate Chemistry, vol. 30, no. 3, pp. 531–535. https://doi.org/10.1021/acs.bioconjchem.8b00868

Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions. / Walden, Grace; Liao, Xin; Riley, Graham et al.
In: Bioconjugate Chemistry, Vol. 30, No. 3, 20.03.2019, p. 531–535.

Research output: Contribution to journal › Article › peer-review

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T1 - Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions

AU - Walden, Grace

AU - Liao, Xin

AU - Riley, Graham

AU - Donell, Simon

AU - Raxworthy, Michael J.

AU - Saeed, Aram

PY - 2019/3/20

Y1 - 2019/3/20

N2 - The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.

AB - The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.

UR - https://www.scopus.com/pages/publications/85062064792

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DO - 10.1021/acs.bioconjchem.8b00868

M3 - Article

AN - SCOPUS:85062064792

SN - 1043-1802

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SP - 531

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JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

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ER -

Walden G, Liao X, Riley G, Donell S, Raxworthy MJ, Saeed A. Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions. Bioconjugate Chemistry. 2019 Mar 20;30(3):531–535. Epub 2019 Feb 7. doi: 10.1021/acs.bioconjchem.8b00868

Synthesis and fabrication of surface-active microparticles using a membrane emulsion technique and conjugation of model protein via strain-promoted azide–alkyne click chemistry in physiological conditions

Abstract

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