Synthetic Macrocyclic Peptidomimetics as Tunable pH Probes for the Fluorescence Imaging of Acidic Organelles in Live Cells

Francisco Galindo, M. Isabel Burguete, Laura Vigara, Santiago V. Luis, Nurul Kabir, Jelena Gavrilovic, David A. Russell

Research output: Contribution to journalArticlepeer-review

132 Citations (Scopus)

Abstract

Acid test: Macrocyclic compounds that comprise a 9,10-anthracene subunit linked by a C2-symmetric peptidomimetic chain (see formula) are useful as fluorescent probes in a pH range of biomedical interest. Changes in the size of the macrocycle (n=3,4,6,8) produce a shift in the pKa values. Experiments with live mouse macrophage cells show the selective localization of the probe in the acidic organelles (see differential interference contrast image).
Original languageEnglish
Pages (from-to)6504-6508
Number of pages5
JournalAngewandte Chemie-International Edition
Volume44
Issue number40
DOIs
Publication statusPublished - 2005

Cite this