Targeted metagenomics of active microbial populations with stable-isotope probing

Sara Coyotzi; Jennifer Pratscher; J. Colin Murrell; Josh D. Neufeld

doi:10.1016/j.copbio.2016.02.017

Targeted metagenomics of active microbial populations with stable-isotope probing

Sara Coyotzi, Jennifer Pratscher, J. Colin Murrell, Josh D. Neufeld

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Abstract

The ability to explore microbial diversity and function has been enhanced by novel experimental and computational tools. The incorporation of stable isotopes into microbial biomass enables the recovery of labeled nucleic acids from active microorganisms, despite their initial abundance and culturability. Combining stable-isotope probing (SIP) with metagenomics provides access to genomes from microorganisms involved in metabolic processes of interest. Studies using metagenomic analysis on DNA obtained from DNA-SIP incubations can be ideal for the recovery of novel enzymes for biotechnology applications, including biodegradation, biotransformation, and biosynthesis. This chapter introduces metagenomic and DNA-SIP methodologies, highlights biotechnology-focused studies that combine these approaches, and provides perspectives on future uses of these methods as analysis tools for applied and environmental microbiology.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Current Opinion in Biotechnology
Volume	41
Early online date	3 Mar 2016
DOIs	https://doi.org/10.1016/j.copbio.2016.02.017
Publication status	Published - Oct 2016

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10.1016/j.copbio.2016.02.017

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Cite this

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title = "Targeted metagenomics of active microbial populations with stable-isotope probing",

abstract = "The ability to explore microbial diversity and function has been enhanced by novel experimental and computational tools. The incorporation of stable isotopes into microbial biomass enables the recovery of labeled nucleic acids from active microorganisms, despite their initial abundance and culturability. Combining stable-isotope probing (SIP) with metagenomics provides access to genomes from microorganisms involved in metabolic processes of interest. Studies using metagenomic analysis on DNA obtained from DNA-SIP incubations can be ideal for the recovery of novel enzymes for biotechnology applications, including biodegradation, biotransformation, and biosynthesis. This chapter introduces metagenomic and DNA-SIP methodologies, highlights biotechnology-focused studies that combine these approaches, and provides perspectives on future uses of these methods as analysis tools for applied and environmental microbiology.",

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