Techniques for the production, isolation, and analysis of iron-sulfur proteins

Jason C Crack, Jeffrey Green, Andrew J Thomson, Nick E Le Brun

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Iron-sulfur clusters constitute a group of cofactors found in many proteins that play key roles in an exceptionally wide range of metabolic processes. The chemical reactivity of iron-sulfur clusters means that they can be particularly prone to damage when removed from the protective environment of the cell. In general, the key to obtaining an intact, biologically active iron-sulfur cluster-containing protein is to maintain a strictly anaerobic environment throughout the entire process of protein purification and analysis. For many proteins, particularly those with more labile clusters, it is essential.
Original languageEnglish
Pages (from-to)33-48
Number of pages16
JournalMethods in Molecular Biology
Volume1122
DOIs
Publication statusPublished - 2014

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