TY - JOUR
T1 - Temperature-dependent inulin nanoparticles synthesized by Lactobacillus reuteri 121 inulosucrase and complex formation with flavonoids
AU - Charoenwongpaiboon, Thanapon
AU - Wangpaiboon, Karan
AU - Panpetch, Pawinee
AU - Field, Robert A.
AU - Barclay, J. Elaine
AU - Pichyangkura, Rath
AU - Kuttiyawong, Kamontip
N1 - Funding Information: TC is grateful for scholarship support from Science Achievement Scholarship of Thailand (SAST) . KK was partially supported by the Thailand Research Fund (MRG5480232) and Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus (year 2016). Research at the JIC is supported by the UK BBSRC Institute Strategic Program on Molecules from Nature (MfN) [BB/PO12523/1] and the John Innes Foundation.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Inulin nanoparticles (INNPs) are a biocompatible material which has a potential application for enhancing solubility and preventing degradation of compounds. In this work, we demonstrated that INNPs could be synthesized from sucrose using inulosucrase from Lactobacillus reuteri 121. Noticeably, dynamic light scattering (DLS) analysis showed that the derived INNPs exhibited uniformity in size, which was easily controlled by the reaction temperature. The effect of enzyme and sucrose concentration, as well as reaction time, was explored. Moreover, the solubility of INNPs in various organic solvents was also investigated, and we found that the INNPs were freely regenerated in water even though they had precipitated by organic solvents. Essentially, we demonstrated that the derived INNPs could be applied for flavonoid encapsulation. The solubility and stability of quercetin and fisetin in the INNPs complexes was higher than those of free compounds. These results make the INNPs very promising for many applications.
AB - Inulin nanoparticles (INNPs) are a biocompatible material which has a potential application for enhancing solubility and preventing degradation of compounds. In this work, we demonstrated that INNPs could be synthesized from sucrose using inulosucrase from Lactobacillus reuteri 121. Noticeably, dynamic light scattering (DLS) analysis showed that the derived INNPs exhibited uniformity in size, which was easily controlled by the reaction temperature. The effect of enzyme and sucrose concentration, as well as reaction time, was explored. Moreover, the solubility of INNPs in various organic solvents was also investigated, and we found that the INNPs were freely regenerated in water even though they had precipitated by organic solvents. Essentially, we demonstrated that the derived INNPs could be applied for flavonoid encapsulation. The solubility and stability of quercetin and fisetin in the INNPs complexes was higher than those of free compounds. These results make the INNPs very promising for many applications.
KW - Encapsulation
KW - Flavonoids
KW - Inulin nanoparticles
KW - Inulosucrase
UR - http://www.scopus.com/inward/record.url?scp=85068482297&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2019.115044
DO - 10.1016/j.carbpol.2019.115044
M3 - Article
C2 - 31427007
AN - SCOPUS:85068482297
VL - 223
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
M1 - 115044
ER -