Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward

G. A. Toole, G. Le Gall, I. J. Colquhoun, C. Nemeth, L. Saulnier, A. Lovegrove, T. Pellny, M. D. Wilkinson, J. Freeman, R. A.C. Mitchell, E. N.C. Mills, P. R. Shewry

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A combination of enzyme mapping, FT-IR microscopy and NMR spectroscopy was used to study temporal and spatial aspects of endosperm cell wall synthesis and deposition in developing grain of bread wheat cv. Hereward. This confirmed previous reports that changes in the proportions of the two major groups of cell wall polysaccharides occur, with β-glucan accumulating earlier in development than arabinoxylan. Changes in the structure of the arabinoxylan occurred, with decreased proportions of disubstituted xylose residues and increased proportions of monosubstituted xylose residues. These are likely to result, at least in part, from arabinoxylan restructuring catalysed by enzymes such as arabinoxylan arabinofurano hydrolase and lead to changes in cell wall mechanical properties which may be required to withstand stresses during grain maturation and desiccation.

Original languageEnglish
Pages (from-to)677-689
Number of pages13
JournalPlanta
Volume232
Issue number3
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Keywords

  • β-Glucan
  • Arabinoxylan
  • Cell wall
  • Dietary fibre
  • Wheat grain

Cite this