The activated human met gene encodes a protein tyrosine kinase

Philip R. Tempest, Colin S. Cooper, Glenn N. Major

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


We have raised antibodies against a synthetic dodecapeptide corresponding to the carboxyl terminus of the predicted met gene product. Phosphorylation of 60 kDa and 65 kDa proteins on tyrosine residues was observed when immunoprecipitates of cells containing the activated human met gene were incubated with [γ-32P]ATP. Phosphoproteins with the same molecular masses could be immunoprecipitated from cells metabolically labelled with [32P]orthophosphate. When considered together, these observations indicate that the activated human met gene encodes 60 kDa and 65 kDa proteins that can catalyse autophosphorylation on tyrosine residues.

Original languageEnglish
Pages (from-to)357-361
Number of pages5
JournalFEBS Letters
Issue number2
Publication statusPublished - 15 Dec 1986


  • Antipeptide antibody
  • metoncogene
  • Tyrosine kinase

Cite this