The activation of fibroblast growth factors by heparin: Synthesis and structural study of rationally modified heparin-like oligosaccharides

Rafael Ojeda; Jesús Angulo; Pedro M. Nieto; Manuel Martín-Lomas

doi:10.1139/v02-023

The activation of fibroblast growth factors by heparin: Synthesis and structural study of rationally modified heparin-like oligosaccharides

Rafael Ojeda, Jesús Angulo, Pedro M. Nieto, Manuel Martín-Lomas

Pharmaceutical Materials and Soft Matter

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Heparin-like hexasaccharide 3 and octasaccharide 4 have been synthesized using a convergent block strategy and their solution conformations have been determined by NMR spectroscopy. Both oligosaccharides contain the basic structural motif of the regular region of heparin but have been constructed as to display negatively charged sulfate groups only on one side of their solution helical structures. This charge distribution along the saccharide chain has been designed to get insight into the proposed mechanism for fibroblast growth factors (FGFs) activation that involves heparin-induced FGF dimerization.

Original language	English
Pages (from-to)	917-936
Number of pages	20
Journal	Canadian Journal of Chemistry (CJC)
Volume	80
Issue number	8
DOIs	https://doi.org/10.1139/v02-023
Publication status	Published - 1 Aug 2002

Keywords

heparin oligosaccharides
synthesis design
conformational analysis
FGF activation

Access to Document

10.1139/v02-023

Cite this

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abstract = "Heparin-like hexasaccharide 3 and octasaccharide 4 have been synthesized using a convergent block strategy and their solution conformations have been determined by NMR spectroscopy. Both oligosaccharides contain the basic structural motif of the regular region of heparin but have been constructed as to display negatively charged sulfate groups only on one side of their solution helical structures. This charge distribution along the saccharide chain has been designed to get insight into the proposed mechanism for fibroblast growth factors (FGFs) activation that involves heparin-induced FGF dimerization.",

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AU - Angulo, Jesús

AU - Nieto, Pedro M.

AU - Martín-Lomas, Manuel

PY - 2002/8/1

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N2 - Heparin-like hexasaccharide 3 and octasaccharide 4 have been synthesized using a convergent block strategy and their solution conformations have been determined by NMR spectroscopy. Both oligosaccharides contain the basic structural motif of the regular region of heparin but have been constructed as to display negatively charged sulfate groups only on one side of their solution helical structures. This charge distribution along the saccharide chain has been designed to get insight into the proposed mechanism for fibroblast growth factors (FGFs) activation that involves heparin-induced FGF dimerization.

AB - Heparin-like hexasaccharide 3 and octasaccharide 4 have been synthesized using a convergent block strategy and their solution conformations have been determined by NMR spectroscopy. Both oligosaccharides contain the basic structural motif of the regular region of heparin but have been constructed as to display negatively charged sulfate groups only on one side of their solution helical structures. This charge distribution along the saccharide chain has been designed to get insight into the proposed mechanism for fibroblast growth factors (FGFs) activation that involves heparin-induced FGF dimerization.

KW - heparin oligosaccharides

KW - synthesis design

KW - conformational analysis

KW - FGF activation

U2 - 10.1139/v02-023

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M3 - Article

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