The antimycobacterial components of hops (Humulus lupulus) and their dereplication

Michael Stavri, Ruth Schneider, Gemma O'Donnell, Doris Lechner, Franz Bucar, Simon Gibbons

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Bioassay‐guided fractionation of a hexane extract of strobile hops (Humulus lupulus ) was undertaken to isolate and characterize the antimycobacterial constituents using the fast‐growing mycobacterial species Mycobacterium fortuitum . Activity was associated with a low polarity fraction and 1H NMR spectra indicated the presence of a fatty acid mixture with unsaturated components. GC‐MS of the derivatives indicated the presence of palmitic, stearic and oleic acids with small quantities of lignoceric, arachidic, behenic and linoleic acids. These compounds were assessed against M. fortuitum and all saturated fatty acids were inactive at concentrations greater than 256 µg/ml, whereas the unsaturated fats oleic and linoleic acids displayed minimum inhibitory concentrations of between 4 and 16 µg/ml against the fast‐growing species tested. The widespread occurrence of these components could render screening for antimycobacterials from natural sources highly problematic without adequate dereplication. We propose that GC‐MS of derivatised components of lipophilic extracts be a first step before any antimycobacterial bioassay‐guided study, as this technique is the method of choice for dereplication of fatty acids.
Original languageEnglish
Pages (from-to)774-776
Number of pages3
JournalPhytotherapy Research
Issue number9
Publication statusPublished - 1 Sep 2004

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