The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in Escherichia coli

James W. Donald; Matthew G. Hicks; David J. Richardson; Tracy Palmer

doi:10.1128/JB.00395-08

The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in Escherichia coli

James W. Donald, Matthew G. Hicks, David J. Richardson, Tracy Palmer

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

Original language	English
Pages (from-to)	5127-5131
Number of pages	5
Journal	Journal of Bacteriology
Volume	190
Issue number	14
DOIs	https://doi.org/10.1128/JB.00395-08
Publication status	Published - Jul 2008

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10.1128/JB.00395-08

Cite this

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title = "The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in Escherichia coli",

abstract = "We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.",

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AU - Hicks, Matthew G.

AU - Richardson, David J.

AU - Palmer, Tracy

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N2 - We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

AB - We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

U2 - 10.1128/JB.00395-08

DO - 10.1128/JB.00395-08

M3 - Article

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SP - 5127

EP - 5131

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

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