TY - JOUR
T1 - The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity
AU - Thor, Kathrin
AU - Jiang, Shushu
AU - Michard, Erwan
AU - George, Jeoffrey
AU - Scherzer, Sönke
AU - Huang, Shouguang
AU - Dindas, Julian
AU - Derbyshire, Paul
AU - Leitão, Nuno
AU - Defalco, Thomas A.
AU - Köster, Philipp
AU - Hunter, Kerri
AU - Kimura, Sachie
AU - Gronnier, Julien
AU - Stransfeld, Lena
AU - Kadota, Yasuhiro
AU - Bücherl, Christoph A.
AU - Charpentier, Myriam
AU - Wrzaczek, Michael
AU - Maclean, Daniel
AU - Oldroyd, Giles E. D.
AU - Menke, Frank L. H.
AU - Roelfsema, M. Rob G.
AU - Hedrich, Rainer
AU - Feijó, José
AU - Zipfel, Cyril
N1 - Publisher Correction: 10.1038/s41586-020-2954-9
PY - 2020/9/24
Y1 - 2020/9/24
N2 - Perception of biotic and abiotic stresses often leads to stomatal closure in plants
1,2. Rapid influx of calcium ions (Ca
2+) across the plasma membrane has an important role in this response, but the identity of the Ca
2+ channels involved has remained elusive
3,4. Here we report that the Arabidopsis thaliana Ca
2+-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca
2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid—a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca
2+ channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca
2+ influx mechanisms in response to different stresses.
AB - Perception of biotic and abiotic stresses often leads to stomatal closure in plants
1,2. Rapid influx of calcium ions (Ca
2+) across the plasma membrane has an important role in this response, but the identity of the Ca
2+ channels involved has remained elusive
3,4. Here we report that the Arabidopsis thaliana Ca
2+-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca
2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid—a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca
2+ channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca
2+ influx mechanisms in response to different stresses.
UR - http://www.scopus.com/inward/record.url?scp=85089865069&partnerID=8YFLogxK
U2 - 10.1038/s41586-020-2702-1
DO - 10.1038/s41586-020-2702-1
M3 - Article
SN - 0028-0836
VL - 585
SP - 569
EP - 573
JO - Nature
JF - Nature
IS - 7826
ER -