The collagen synthesis response to an acute bout of resistance exercise is greater when ingesting 30 g versus 15 g and 0 g hydrolyzed collagen in resistance-trained young men

Joonsung Lee, Jonathan C. Y. Tang, John Dutton, Rachel Dunn, William D. Fraser, Kevin Enright, David R. Clark, Claire E. Stewart, Robert M. Erskine (Lead Author)

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Background: Resistance exercise (RE) stimulates collagen synthesis in skeletal muscle and tendon but there is limited and equivocal evidence regarding an effect of collagen supplementation and exercise on collagen synthesis. Furthermore, it is not known if a dose–response exists regarding the effect of hydrolyzed collagen (HC) ingestion and RE on collagen synthesis. Objective: To determine the HC dose–response effect on collagen synthesis after high-intensity RE in resistance-trained young men. Methods: Using a double-blind, randomized crossover design, 10 resistance-trained males (age: 26 ± 3 y; height: 1.77 ± 0.04 m; mass: 79.7 ± 7.0 kg) ingested 0 g, 15 g, or 30 g HC with 50 mg vitamin C 1 h before performing 4 sets’ barbell back squat RE at 10-repetition maximum load, after which they rested for 6 h. Blood samples were collected throughout each of the 3 interventions to analyze procollagen type Ⅰ N-terminal propeptide (PINP) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX) concentration, and the concentration of 18 collagen amino acids. Results: The serum PINP concentration × time area under the curve (AUC) was greater for 30 g (267 ± 79 μg·L −1·h) than for 15 g (235 ± 70 μg·L −1·h, P = 0.013) and 0 g HC (219 ± 88 μg·L −1·h, P = 0.002) but there was no difference between 0 and 15 g HC (P = 0.225). The AUCs of glycine and proline were greater for 30 g than for 15 and 0 g HC (P < 0.05). Plasma β-CTX concentration decreased from −1 to +6 h (P < 0.05), with no differences between interventions. Conclusions: Ingesting 30 g HC before high-intensity RE augments whole-body collagen synthesis more than 15 g and 0 g HC in resistance-trained young males.

Original languageEnglish
JournalThe Journal of Nutrition
Early online date24 Nov 2023
Publication statusE-pub ahead of print - 24 Nov 2023


  • connective tissue
  • glycine
  • hydroxyproline
  • proline
  • strength training
  • vitamin C

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