The divergent restoration effects of Lactobacillus strains in antibiotic-induced dysbiosis

Ying Shi, Lee Kellingray, Gwenaelle Le Gall, Jianxin Zhao, Hao Zhang, Arjan Narbad, Qixiao Zhai, Wei Chen

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)
17 Downloads (Pure)


To evaluate functions of Lactobacillus strains, isolated from fermented food, in restoration of ampicillin-induced disruption based on mucosal barrier, gut microbial community and metabolome analyses, three Lactobacillus strains, L. plantarum CGMCC12436 (LacP), L. casei CGMCC 12,435 (LacC) and L. rhamnosus strain GG (LacG) were individually administered to ampicillin-pretreated mice. All three strains significantly restored concentrations of endotoxin and diamine oxidase to control levels. Linear discriminate analysis based on 16S rRNA sequencing of faecal bacteria revealed that the restoration of microbial communities by Lactobacillus strains was more effective than natural restoration. Correlation analysis between microbiota and metabolites indicated that, the higher level of acetate in LacC group was positively correlated with increased relative abundance of Citrobacter, Bifidobacterium and S24-7. Furthermore, LacC down-regulated the expression of NF-κB p65 and modulated the ampicillin-induced inflammatory responses. The LacC strain could particularly attenuate ampicillin-induced disruption by optimisation of microbial taxa and enhancement of acetate and butyrate production.

Original languageEnglish
Pages (from-to)142-152
Number of pages11
JournalJournal of Functional Foods
Early online date29 Oct 2018
Publication statusPublished - Dec 2018
Externally publishedYes


  • Functional food
  • Gut dysbiosis
  • Immunity
  • Metabolites
  • Microbial ecology

Cite this