TY - JOUR
T1 - The effect of reserpine, a modulator of multidrug efflux pumps, on the in vitro activity of tetracycline against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) possessing the tet(K) determinant
AU - Gibbons, Simon
AU - Udo, E. E.
PY - 2000/3
Y1 - 2000/3
N2 - As part of a screening programme to identify modulators of multidrug efflux in methicillin resistant Staphyloccocus aureus (MRSA), we have validated our assays using the antihypertensive plant alkaloid reserpine. Clinical isolates of MRSA were resistant to tetracycline and shown to possess the tet(K) determinant which encodes for the Tet(K) efflux protein, which conferred high level resistance to tetracycline (MIC = 128 μg/mL). In the presence of reserpine, a known inhibitor of multidrug resistance (mdr) efflux pumps, this MIC was significantly reduced (MIC = 32 μg/mL). Copyright (C) 2000 John Wiley and Sons, Ltd.
AB - As part of a screening programme to identify modulators of multidrug efflux in methicillin resistant Staphyloccocus aureus (MRSA), we have validated our assays using the antihypertensive plant alkaloid reserpine. Clinical isolates of MRSA were resistant to tetracycline and shown to possess the tet(K) determinant which encodes for the Tet(K) efflux protein, which conferred high level resistance to tetracycline (MIC = 128 μg/mL). In the presence of reserpine, a known inhibitor of multidrug resistance (mdr) efflux pumps, this MIC was significantly reduced (MIC = 32 μg/mL). Copyright (C) 2000 John Wiley and Sons, Ltd.
KW - Efflux
KW - Mdr
KW - MRSA
KW - Multidrug resistance
KW - Tet(K)
UR - http://www.scopus.com/inward/record.url?scp=0034024754&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1099-1573(200003)14:2<139::AID-PTR608>3.0.CO;2-8
DO - 10.1002/(SICI)1099-1573(200003)14:2<139::AID-PTR608>3.0.CO;2-8
M3 - Article
C2 - 10685116
AN - SCOPUS:0034024754
VL - 14
SP - 139
EP - 140
JO - Phytotherapy Research
JF - Phytotherapy Research
SN - 0951-418X
IS - 2
ER -