The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Desirè Di Silvio; Marco Maccarini; Roger Parker; Alan Mackie; Giovanna Fragneto; Francesca Baldelli Bombelli

doi:10.1016/j.jcis.2017.05.086

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Desirè Di Silvio, Marco Maccarini, Roger Parker, Alan Mackie, Giovanna Fragneto, Francesca Baldelli Bombelli

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

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Abstract

Hypothesis: It is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure.

Experiments: In the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NP-membrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer.

Findings: Our study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon.

Original language	English
Pages (from-to)	741–750
Journal	Journal of Colloid and Interface Science
Volume	504
Early online date	29 May 2017
DOIs	https://doi.org/10.1016/j.jcis.2017.05.086
Publication status	Published - 15 Oct 2017

Keywords

Supported lipid bilayer
Protein corona nanoparticles
Quartz crystal microbalance
Neutron reflectometry
Soft corona
Hard corona

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10.1016/j.jcis.2017.05.086

JCIS_2017Accepted author manuscript, 1.49 MBLicence: CC BY-NC-ND

http://linkinghub.elsevier.com/retrieve/pii/S0021979717306148

Development of multifunctional targeted drug nanocarriers for the treatment of metastatic melanoma
Baldelli Bombelli, F. & Sherwood, V.
British Skin Foundation (The)
1/01/13 → 31/12/14
Project: Research
Development of SPION based theranostic drug nanocarriers for the treatment of multi drug resistance cancer cells
Baldelli Bombelli, F.
Royal Society
1/04/12 → 31/03/13
Project: Research

Cite this

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title = "The effect of the protein corona on the interaction between nanoparticles and lipid bilayers",

abstract = "Hypothesis: It is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. Experiments: In the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NP-membrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. Findings: Our study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon.",

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author = "Silvio, {Desir{\`e} Di} and Marco Maccarini and Roger Parker and Alan Mackie and Giovanna Fragneto and Bombelli, {Francesca Baldelli}",

year = "2017",

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doi = "10.1016/j.jcis.2017.05.086",

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T1 - The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

AU - Silvio, Desirè Di

AU - Maccarini, Marco

AU - Parker, Roger

AU - Mackie, Alan

AU - Fragneto, Giovanna

AU - Bombelli, Francesca Baldelli

PY - 2017/10/15

Y1 - 2017/10/15

N2 - Hypothesis: It is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. Experiments: In the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NP-membrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. Findings: Our study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon.

AB - Hypothesis: It is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. Experiments: In the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NP-membrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. Findings: Our study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon.

KW - Supported lipid bilayer

KW - Protein corona nanoparticles

KW - Quartz crystal microbalance

KW - Neutron reflectometry

KW - Soft corona

KW - Hard corona

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DO - 10.1016/j.jcis.2017.05.086

M3 - Article

VL - 504

SP - 741

EP - 750

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Abstract

Keywords

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Projects

Development of multifunctional targeted drug nanocarriers for the treatment of metastatic melanoma

Development of SPION based theranostic drug nanocarriers for the treatment of multi drug resistance cancer cells

Cite this