The effect of trans-10, cis-12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal-like Caco-2 cells

Eileen F Murphy; Guido J Hooiveld; Michael Müller; Raffaelle A Calogero; Kevin D Cashman

doi:10.1007/s12263-009-0116-7

The effect of trans-10, cis-12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal-like Caco-2 cells

Eileen F Murphy, Guido J Hooiveld, Michael Müller, Raffaelle A Calogero, Kevin D Cashman

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We conducted an in-depth investigation of the effects of conjugated linoleic acid (CLA) on the expression of key metabolic genes and genes of known importance in intestinal lipid metabolism using the Caco-2 cell model. Cells were treated with 80 mumol/L of linoleic acid (control), trans-10, cis-12 CLA or cis-9, trans-11 CLA. RNA was isolated from the cells, labelled and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis were preformed using Bioconductor. Gene ontology analysis (GO) revealed a significant enrichment (P

Original language	English
Pages (from-to)	103-112
Number of pages	10
Journal	Genes & Nutrition
Volume	4
Issue number	2
DOIs	https://doi.org/10.1007/s12263-009-0116-7
Publication status	Published - Jun 2009

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10.1007/s12263-009-0116-7

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title = "The effect of trans-10, cis-12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal-like Caco-2 cells",

abstract = "We conducted an in-depth investigation of the effects of conjugated linoleic acid (CLA) on the expression of key metabolic genes and genes of known importance in intestinal lipid metabolism using the Caco-2 cell model. Cells were treated with 80 mumol/L of linoleic acid (control), trans-10, cis-12 CLA or cis-9, trans-11 CLA. RNA was isolated from the cells, labelled and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis were preformed using Bioconductor. Gene ontology analysis (GO) revealed a significant enrichment (P ",

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AU - Calogero, Raffaelle A

AU - Cashman, Kevin D

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