The effect of trans-10, cis-12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal-like Caco-2 cells

Eileen F Murphy, Guido J Hooiveld, Michael Müller, Raffaelle A Calogero, Kevin D Cashman

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8 Citations (Scopus)


We conducted an in-depth investigation of the effects of conjugated linoleic acid (CLA) on the expression of key metabolic genes and genes of known importance in intestinal lipid metabolism using the Caco-2 cell model. Cells were treated with 80 mumol/L of linoleic acid (control), trans-10, cis-12 CLA or cis-9, trans-11 CLA. RNA was isolated from the cells, labelled and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis were preformed using Bioconductor. Gene ontology analysis (GO) revealed a significant enrichment (P
Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalGenes & Nutrition
Issue number2
Publication statusPublished - Jun 2009

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