The genome of Clostridium difficile 5.3

Aaron E Darling, Paul Worden, Toni A Chapman, Piklu Chowdhury, Ian G Charles, Steven P Djordjevic

Research output: Contribution to journalArticle

10 Citations (Scopus)
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Abstract

Background
Clostridium difficile is the leading cause of infectious diarrhea in humans and responsible for large outbreaks of enteritis in neonatal pigs in both North America and Europe. Disease caused by C. difficile typically occurs during antibiotic therapy and its emergence over the past 40 years is linked with the widespread use of broad-spectrum antibiotics in both human and veterinary medicine.

Results
We sequenced the genome of Clostridium difficile 5.3 using the Illumina Nextera XT and MiSeq technologies. Assembly of the sequence data reconstructed a 4,009,318 bp genome in 27 scaffolds with an N50 of 786 kbp. The genome has extensive similarity to other sequenced C. difficile genomes, but also has several genes that are potentially related to virulence and pathogenicity that are not present in the reference C. difficile strain.

Conclusion
Genome sequencing of human and animal isolates is needed to understand the molecular events driving the emergence of C. difficile as a gastrointestinal pathogen of humans and food animals and to better define its zoonotic potential.
Original languageEnglish
Article number4
JournalGut Pathogens
Volume6
Issue number1
DOIs
Publication statusPublished - 24 Feb 2014

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