The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria

Lucie Trdá, Olivier Fernandez, Freddy Boutrot, Marie-Claire Héloir, Jani Kelloniemi, Xavier Daire, Marielle Adrian, Christophe Clément, Cyril Zipfel, Stéphan Dorey, Benoit Poinssot

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

The role of flagellin perception in the context of plant beneficial bacteria still remains unclear. Here, we characterized the flagellin sensing system flg22-FLAGELLIN SENSING 2 (FLS2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (PGPR) Burkholderia phytofirmans. • The functionality of the grapevine FLS2 receptor, VvFLS2, was demonstrated by complementation assays in the Arabidopsis thaliana fls2 mutant, which restored flg22-induced H₂O₂ production and growth inhibition. Using synthetic flg22 peptides from different bacterial origins, we compared recognition specificities between VvFLS2 and AtFLS2. • In grapevine, flg22-triggered immune responses are conserved and led to partial resistance against Botrytis cinerea. Unlike flg22 peptides derived from Pseudomonas aeruginosa or Xanthomonas campestris, flg22 peptide derived from B. phytofirmans triggered only a small oxidative burst, weak and transient defense gene induction and no growth inhibition in grapevine. Although, in Arabidopsis, all the flg22 epitopes exhibited similar biological activities, the expression of VvFLS2 into the fls2 background conferred differential flg22 responses characteristic for grapevine. • These results demonstrate that VvFLS2 differentially recognizes flg22 from different bacteria, and suggest that flagellin from the beneficial PGPR B. phytofirmans has evolved to evade this grapevine immune recognition system.
Original languageEnglish
Pages (from-to)1371-1384
Number of pages14
JournalNew Phytologist
Volume201
Issue number4
DOIs
Publication statusPublished - Mar 2014

Keywords

  • Burkholderia phytofirmans
  • flagellin sensing
  • flg22
  • microbe-associated molecular pattern (MAMP)
  • pattern recognition receptor (PRR)
  • PGPR
  • Vitis vinifera

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