The identification of a periplasmic nitrate reductase in Paracoccus denitrificans

Heather J. Sears; Stuart J. Ferguson; David J. Richardson; Stephen Spiro

doi:10.1111/j.1574-6968.1993.tb06496.x

The identification of a periplasmic nitrate reductase in Paracoccus denitrificans

Heather J. Sears, Stuart J. Ferguson, David J. Richardson, Stephen Spiro

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans. The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon.

Original language	English
Pages (from-to)	107-111
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	113
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06496.x
Publication status	Published - 1 Oct 1993

Keywords

Aerobic denitrification
Nitrate reductase
Paracoccus denitrificans
Periplasm

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10.1111/j.1574-6968.1993.tb06496.x

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abstract = "A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans. The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon.",

keywords = "Aerobic denitrification, Nitrate reductase, Paracoccus denitrificans, Periplasm",

author = "Sears, {Heather J.} and Ferguson, {Stuart J.} and Richardson, {David J.} and Stephen Spiro",

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AU - Richardson, David J.

AU - Spiro, Stephen

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AB - A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans. The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon.

KW - Aerobic denitrification

KW - Nitrate reductase

KW - Paracoccus denitrificans

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The identification of a periplasmic nitrate reductase in Paracoccus denitrificans

Abstract

Keywords

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