The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis

Ayesha Azeem, Andrew English, Pramod Kumar, Abhigyan Satyam, Manus Biggs, Eleanor Jones, Bhawana Tripathi, Nandita Basu, Jan Henkel, Cedryck Vaquette, Niall Rooney, Graham Riley, Alan O'Riordan, Graham Cross, Saso Ivanovski, Dietmar Hutmacher, Abhay Pandit, Dimitrios Zeugolis

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

AIM: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell-substrate interface.

MATERIALS & METHODS: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ~1860 nm; constant line width of ~2220 nm; variable groove depth of ~35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed.

RESULTS & DISCUSSION: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo.

CONCLUSION: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.

Original languageEnglish
Pages (from-to)693-711
Number of pages19
JournalNanomedicine
Volume10
Issue number5
DOIs
Publication statusPublished - 2015

Keywords

  • anisotropic topography
  • cell/biomaterial interface
  • gene analysis
  • osteogenesis
  • MESENCHYMAL STEM-CELLS
  • TISSUE ENGINEERING APPLICATIONS
  • PERIPHERAL-NERVE REPAIR
  • FOCAL ADHESION KINASE
  • REGENERATIVE MEDICINE
  • DRUG-DELIVERY
  • OSTEOBLAST ALIGNMENT
  • CONTROLLED-RELEASE
  • SOFT-LITHOGRAPHY
  • CELLULAR MECHANOTRANSDUCTION

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