The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis

Ayesha Azeem; Andrew English; Pramod Kumar; Abhigyan Satyam; Manus Biggs; Eleanor Jones; Bhawana Tripathi; Nandita Basu; Jan Henkel; Cedryck Vaquette; Niall Rooney; Graham Riley; Alan O'Riordan; Graham Cross; Saso Ivanovski; Dietmar Hutmacher; Abhay Pandit; Dimitrios Zeugolis

doi:10.2217/nnm.14.218

The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis

Ayesha Azeem, Andrew English, Pramod Kumar, Abhigyan Satyam, Manus Biggs, Eleanor Jones, Bhawana Tripathi, Nandita Basu, Jan Henkel, Cedryck Vaquette, Niall Rooney, Graham Riley, Alan O'Riordan, Graham Cross, Saso Ivanovski, Dietmar Hutmacher, Abhay Pandit, Dimitrios Zeugolis

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

AIM: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell-substrate interface.

MATERIALS & METHODS: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ~1860 nm; constant line width of ~2220 nm; variable groove depth of ~35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed.

RESULTS & DISCUSSION: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo.

CONCLUSION: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.

Original language	English
Pages (from-to)	693-711
Number of pages	19
Journal	Nanomedicine
Volume	10
Issue number	5
DOIs	https://doi.org/10.2217/nnm.14.218
Publication status	Published - 2015

Keywords

anisotropic topography
cell/biomaterial interface
gene analysis
osteogenesis
MESENCHYMAL STEM-CELLS
TISSUE ENGINEERING APPLICATIONS
PERIPHERAL-NERVE REPAIR
FOCAL ADHESION KINASE
REGENERATIVE MEDICINE
DRUG-DELIVERY
OSTEOBLAST ALIGNMENT
CONTROLLED-RELEASE
SOFT-LITHOGRAPHY
CELLULAR MECHANOTRANSDUCTION

Access to Document

10.2217/nnm.14.218

Cite this

Azeem, A., English, A., Kumar, P., Satyam, A., Biggs, M., Jones, E., Tripathi, B., Basu, N., Henkel, J., Vaquette, C., Rooney, N., Riley, G., O'Riordan, A., Cross, G., Ivanovski, S., Hutmacher, D., Pandit, A., & Zeugolis, D. (2015). The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis. Nanomedicine, 10(5), 693-711. https://doi.org/10.2217/nnm.14.218

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abstract = "AIM: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell-substrate interface.MATERIALS & METHODS: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ~1860 nm; constant line width of ~2220 nm; variable groove depth of ~35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed.RESULTS & DISCUSSION: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo.CONCLUSION: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.",

keywords = "anisotropic topography, cell/biomaterial interface, gene analysis, osteogenesis, MESENCHYMAL STEM-CELLS, TISSUE ENGINEERING APPLICATIONS, PERIPHERAL-NERVE REPAIR, FOCAL ADHESION KINASE, REGENERATIVE MEDICINE, DRUG-DELIVERY, OSTEOBLAST ALIGNMENT, CONTROLLED-RELEASE, SOFT-LITHOGRAPHY, CELLULAR MECHANOTRANSDUCTION",

author = "Ayesha Azeem and Andrew English and Pramod Kumar and Abhigyan Satyam and Manus Biggs and Eleanor Jones and Bhawana Tripathi and Nandita Basu and Jan Henkel and Cedryck Vaquette and Niall Rooney and Graham Riley and Alan O'Riordan and Graham Cross and Saso Ivanovski and Dietmar Hutmacher and Abhay Pandit and Dimitrios Zeugolis",

year = "2015",

doi = "10.2217/nnm.14.218",

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Azeem, A, English, A, Kumar, P, Satyam, A, Biggs, M, Jones, E, Tripathi, B, Basu, N, Henkel, J, Vaquette, C, Rooney, N, Riley, G, O'Riordan, A, Cross, G, Ivanovski, S, Hutmacher, D, Pandit, A & Zeugolis, D 2015, 'The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis', Nanomedicine, vol. 10, no. 5, pp. 693-711. https://doi.org/10.2217/nnm.14.218

TY - JOUR

T1 - The influence of anisotropic nano- to micro-topography on in vitro and in vivo osteogenesis

AU - Azeem, Ayesha

AU - English, Andrew

AU - Kumar, Pramod

AU - Satyam, Abhigyan

AU - Biggs, Manus

AU - Jones, Eleanor

AU - Tripathi, Bhawana

AU - Basu, Nandita

AU - Henkel, Jan

AU - Vaquette, Cedryck

AU - Rooney, Niall

AU - Riley, Graham

AU - O'Riordan, Alan

AU - Cross, Graham

AU - Ivanovski, Saso

AU - Hutmacher, Dietmar

AU - Pandit, Abhay

AU - Zeugolis, Dimitrios

PY - 2015

Y1 - 2015

N2 - AIM: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell-substrate interface.MATERIALS & METHODS: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ~1860 nm; constant line width of ~2220 nm; variable groove depth of ~35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed.RESULTS & DISCUSSION: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo.CONCLUSION: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.

AB - AIM: Topographically modified substrates are increasingly used in tissue engineering to enhance biomimicry. The overarching hypothesis is that topographical cues will control cellular response at the cell-substrate interface.MATERIALS & METHODS: The influence of anisotropically ordered poly(lactic-co-glycolic acid) substrates (constant groove width of ~1860 nm; constant line width of ~2220 nm; variable groove depth of ~35, 306 and 2046 nm) on in vitro and in vivo osteogenesis were assessed.RESULTS & DISCUSSION: We demonstrate that substrates with groove depths of approximately 306 and 2046 nm promote osteoblast alignment parallel to underlined topography in vitro. However, none of the topographies assessed promoted directional osteogenesis in vivo.CONCLUSION: 2D imprinting technologies are useful tools for in vitro cell phenotype maintenance.

KW - anisotropic topography

KW - cell/biomaterial interface

KW - gene analysis

KW - osteogenesis

KW - MESENCHYMAL STEM-CELLS

KW - TISSUE ENGINEERING APPLICATIONS

KW - PERIPHERAL-NERVE REPAIR

KW - FOCAL ADHESION KINASE

KW - REGENERATIVE MEDICINE

KW - DRUG-DELIVERY

KW - OSTEOBLAST ALIGNMENT

KW - CONTROLLED-RELEASE

KW - SOFT-LITHOGRAPHY

KW - CELLULAR MECHANOTRANSDUCTION

U2 - 10.2217/nnm.14.218

DO - 10.2217/nnm.14.218

M3 - Article

C2 - 25816874

VL - 10

SP - 693

EP - 711

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 5

ER -