The measurement of beclomethasone dipropionate entrapment in liposomes: a comparison of a microscope and an HPLC method

R Batavia; KMG Taylor; DQM Craig; M Thomas

doi:10.1016/S0378-5173(00)00584-6

The measurement of beclomethasone dipropionate entrapment in liposomes: a comparison of a microscope and an HPLC method

R Batavia, KMG Taylor, DQM Craig, M Thomas

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23 Citations (Scopus)

Abstract

The purpose of this study was to examine the methodologies that may be used to estimate the maximum incorporation (<5 mole% drug) of beclomethasone dipropionate (BDP) in dipalmitoylphosphatidylcholine (DPPC) multilamellar liposomes. Two approaches are described. First, differential interference contrast (DIC) microscopy and cross-polarisation microscopy have been used to measure the concentration at which BDP crystals become apparent in BDP-containing liposome preparations, thereby allowing a semi-quantitative but simple estimation of entrapment. An alternative method is described whereby the unentrapped solid drug is separated from the liposomes via suspension in D2O, followed by centrifugation and HPLC analysis. The method resulted in an estimate of 1.5–2 mole% BDP, while the HPLC method yielded a value of 2.52 mole% BDP.

Original language	English
Pages (from-to)	109-119
Number of pages	11
Journal	International Journal of Pharmaceutical Medicine
Volume	212
Issue number	1
DOIs	https://doi.org/10.1016/S0378-5173(00)00584-6
Publication status	Published - 6 Feb 2001

Keywords

HPLC
Crystal
Beclomethasone dipropionate
Microscopy
Liposome

Access to Document

10.1016/S0378-5173(00)00584-6

Cite this

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title = "The measurement of beclomethasone dipropionate entrapment in liposomes: a comparison of a microscope and an HPLC method",

abstract = "The purpose of this study was to examine the methodologies that may be used to estimate the maximum incorporation (<5 mole% drug) of beclomethasone dipropionate (BDP) in dipalmitoylphosphatidylcholine (DPPC) multilamellar liposomes. Two approaches are described. First, differential interference contrast (DIC) microscopy and cross-polarisation microscopy have been used to measure the concentration at which BDP crystals become apparent in BDP-containing liposome preparations, thereby allowing a semi-quantitative but simple estimation of entrapment. An alternative method is described whereby the unentrapped solid drug is separated from the liposomes via suspension in D2O, followed by centrifugation and HPLC analysis. The method resulted in an estimate of 1.5–2 mole% BDP, while the HPLC method yielded a value of 2.52 mole% BDP.",

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T1 - The measurement of beclomethasone dipropionate entrapment in liposomes: a comparison of a microscope and an HPLC method

AU - Batavia, R

AU - Taylor, KMG

AU - Craig, DQM

AU - Thomas, M

PY - 2001/2/6

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N2 - The purpose of this study was to examine the methodologies that may be used to estimate the maximum incorporation (<5 mole% drug) of beclomethasone dipropionate (BDP) in dipalmitoylphosphatidylcholine (DPPC) multilamellar liposomes. Two approaches are described. First, differential interference contrast (DIC) microscopy and cross-polarisation microscopy have been used to measure the concentration at which BDP crystals become apparent in BDP-containing liposome preparations, thereby allowing a semi-quantitative but simple estimation of entrapment. An alternative method is described whereby the unentrapped solid drug is separated from the liposomes via suspension in D2O, followed by centrifugation and HPLC analysis. The method resulted in an estimate of 1.5–2 mole% BDP, while the HPLC method yielded a value of 2.52 mole% BDP.

AB - The purpose of this study was to examine the methodologies that may be used to estimate the maximum incorporation (<5 mole% drug) of beclomethasone dipropionate (BDP) in dipalmitoylphosphatidylcholine (DPPC) multilamellar liposomes. Two approaches are described. First, differential interference contrast (DIC) microscopy and cross-polarisation microscopy have been used to measure the concentration at which BDP crystals become apparent in BDP-containing liposome preparations, thereby allowing a semi-quantitative but simple estimation of entrapment. An alternative method is described whereby the unentrapped solid drug is separated from the liposomes via suspension in D2O, followed by centrifugation and HPLC analysis. The method resulted in an estimate of 1.5–2 mole% BDP, while the HPLC method yielded a value of 2.52 mole% BDP.

KW - HPLC

KW - Crystal

KW - Beclomethasone dipropionate

KW - Microscopy

KW - Liposome

U2 - 10.1016/S0378-5173(00)00584-6

DO - 10.1016/S0378-5173(00)00584-6

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VL - 212

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EP - 119

JO - International Journal of Pharmaceutical Medicine

JF - International Journal of Pharmaceutical Medicine

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