The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification

Victoria Hatch, Marta Marin Barba, Simon Moxon, Christopher Ford, Nicole Ward, Matthew Tomlinson, Ines Desanlis, Adam Hendry, Saartje Hontelez, Ila van Krujsbergen, Gert Jan Veenstra, Andrea Munsterberg, Grant Wheeler

Research output: Contribution to journalArticle

8 Citations (Scopus)
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Abstract

Regulation of gene expression at the level of transcriptional elongation has been shown to be important in stem cells and tumour cells, but its role in the whole animal is only now being fully explored. Neural crest cells (NCCs) are a multipotent population of cells that migrate during early development from the dorsal neural tube throughout the embryo where they differentiate into a variety of cell types including pigment cells, cranio-facial skeleton and sensory neurons. Specification of NCCs is both spatially and temporally regulated during embryonic development. Here we show that components of the transcriptional elongation regulatory machinery, CDK9 and CYCLINT1 of the P-TEFb complex, are required to regulate neural crest specification. In particular, we show that expression of the proto-oncogene c-Myc and c-Myc responsive genes are affected. Our data suggest that P-TEFb is crucial to drive expression of c-Myc, which acts as a ‘gate-keeper’ for the correct temporal and spatial development of the neural crest.
Original languageEnglish
Pages (from-to)361–372
JournalDevelopmental Biology
Volume416
Issue number2
Early online date23 Jun 2016
DOIs
Publication statusPublished - 15 Aug 2016

Keywords

  • Neural crest cells
  • leflunomide
  • transcriptional elongation
  • c-Myc
  • P-TEFb
  • Xenopus
  • polymerase pausing
  • Cdk9
  • CyclinT1

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