TY - JOUR
T1 - The receptor kinase FERONIA regulates phosphatidylserine localization at the cell surface to modulate ROP signaling
AU - Smokvarska, Marija
AU - Bayle, Vincent
AU - Maneta-Peyret, Lilly
AU - Fouillen, Laetitia
AU - Poitout, Arthur
AU - Dongois, Armelle
AU - Fiche, Jean-Bernard
AU - Gronnier, Julien
AU - Garcia, José
AU - Höfte, Herman
AU - Nolmann, Marcelo
AU - Zipfel, Cyril
AU - Maurel, Christophe
AU - Moreau, Patrick
AU - Jaillais, Yvon
AU - Martiniere, Alexandre
N1 - Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Arabidopsis lines generated in this study are deposited to the Eurasian Arabidopsis Stock Center.
Funding Information: J.G. was funded by a Long-Term Fellowships from the European Molecular Biology Organization (EMBO LTF number 438-2018). J.G. is currently funded by the German Research Foundation (DFG) grant CRC 1101-A09. C.Z. received funding from European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program under the Grant Agreement 773153 (project IMMUNO-PEPTALK). H.H. is founded by the French National Agency ANR Homeowall (ANR-20-CE11-0008-01//C6627). Y.J. received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 101001097) and by ERC no. 3363360-APPL under FP/2007-2013; Y.J. and A.M. by the innovative project iRhobot from the division of “Biologie et Amélioration des Plantes” (BAP) of INRAE A.M. is funded by the French National Agency ANR CellOsmo (ANR-19-CE20-0008-01).
PY - 2023/4/7
Y1 - 2023/4/7
N2 - Cells maintain a constant dialog between the extracellular matrix and their plasma membrane to fine tune signal transduction processes. We found that the receptor kinase FERONIA (FER), which is a proposed cell wall sensor, modulates phosphatidylserine plasma membrane accumulation and nano-organization, a key regulator of Rho GTPase signaling in Arabidopsis. We demonstrate that FER is required for both Rho-of-Plant 6 (ROP6) nano-partitioning at the membrane and downstream production of reactive oxygen species upon hyperosmotic stimulus. Genetic and pharmacological rescue experiments indicate that phosphatidylserine is required for a subset of, but not all, FER functions. Furthermore, application of FER ligand shows that its signaling controls both phosphatidylserine membrane localization and nanodomains formation, which, in turn, tunes ROP6 signaling. Together, we propose that a cell wall–sensing pathway controls via the regulation of membrane phospholipid content, the nano-organization of the plasma membrane, which is an essential cell acclimation to environmental perturbations.
AB - Cells maintain a constant dialog between the extracellular matrix and their plasma membrane to fine tune signal transduction processes. We found that the receptor kinase FERONIA (FER), which is a proposed cell wall sensor, modulates phosphatidylserine plasma membrane accumulation and nano-organization, a key regulator of Rho GTPase signaling in Arabidopsis. We demonstrate that FER is required for both Rho-of-Plant 6 (ROP6) nano-partitioning at the membrane and downstream production of reactive oxygen species upon hyperosmotic stimulus. Genetic and pharmacological rescue experiments indicate that phosphatidylserine is required for a subset of, but not all, FER functions. Furthermore, application of FER ligand shows that its signaling controls both phosphatidylserine membrane localization and nanodomains formation, which, in turn, tunes ROP6 signaling. Together, we propose that a cell wall–sensing pathway controls via the regulation of membrane phospholipid content, the nano-organization of the plasma membrane, which is an essential cell acclimation to environmental perturbations.
UR - http://www.scopus.com/inward/record.url?scp=85151935762&partnerID=8YFLogxK
U2 - 10.1126/sciadv.add4791
DO - 10.1126/sciadv.add4791
M3 - Article
C2 - 37027473
AN - SCOPUS:85151935762
VL - 9
JO - Science Advances
JF - Science Advances
SN - 2375-2548
IS - 14
M1 - eadd4791
ER -