@article{6cff424b788f448a993dddf7121ad215,
title = "The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device",
abstract = "There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.",
author = "Baker, {Alexander N.} and Richards, {Sarah Jane} and Guy, {Collette S.} and Congdon, {Thomas R.} and Muhammad Hasan and Zwetsloot, {Alexander J.} and Angelo Gallo and Lewandowski, {J{\'o}zef R.} and Stansfeld, {Phillip J.} and Anne Straube and Marc Walker and Simona Chessa and Giulia Pergolizzi and Simone Dedola and Field, {Robert A.} and Gibson, {Matthew I.}",
note = "Funding Information: MIG is supported by the ERC (638661) and Royal Society (Industry Fellowship 191037). The BBSRC MIBTP program (BB/M01116X/1) and Iceni Diagnostics Ltd are thanked for support to A.N.B. UoW, EPSRC (EP/R511808/1), and BBSRC (BB/S506783/1) impact acceleration accounts are thanked. A.J.Z. is funded by the MRC DTP (MR/N014294/1). A.S. is a Wellcome Trust Investigator (200870/Z/16/Z). The Leverhulme Trust are thanked for support (RPG-2019-087). P.J.S. thanks Wellcome (208361/Z/17/Z), the MRC (MR/S009213/1), and BBSRC (BB/P01948X/1, BB/R002517/1, and BB/S003339/1). The Warwick Polymer and Electron Microscopy Research Technology Platforms (Y. Han) are acknowledged for the SEC/EM analysis. Dr C. Biggs is thanked for lateral flow device preparation. We sincerely thank the technical and administrative staff of the UoW who enabled our laboratory to remain open during the COVID-19 pandemic. Prof. Peter Scott is thanked for critically reading this manuscript. Publisher Copyright: {\textcopyright} 2020 American Chemical Society. All rights reserved.",
year = "2020",
month = nov,
day = "25",
doi = "10.1021/acscentsci.0c00855",
language = "English",
volume = "6",
pages = "2046--2052",
journal = "ACS Central Science",
issn = "2374-7943",
publisher = "American Chemical Society",
number = "11",
}