The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors

Xia Yan, Bozeng Tang, Lauren S. Ryder, Dan MacLean, Vincent M. Were, Alice Bisola Eseola, Neftaly Cruz-Mireles, Weibin Ma, Andrew J. Foster, Miriam Osés-Ruiz, Nicholas J. Talbot

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

The rice blast fungus Magnaporthe oryzae causes a devastating disease that threatens global rice (Oryza sativa) production. Despite intense study, the biology of plant tissue invasion during blast disease remains poorly understood. Here we report a high-resolution transcriptional profiling study of the entire plant-associated development of the blast fungus. Our analysis revealed major temporal changes in fungal gene expression during plant infection. Pathogen gene expression could be classified into 10 modules of temporally co-expressed genes, providing evidence for the induction of pronounced shifts in primary and secondary metabolism, cell signaling, and transcriptional regulation. A set of 863 genes encoding secreted proteins are differentially expressed at specific stages of infection, and 546 genes named MEP (Magnaporthe effector protein) genes were predicted to encode effectors. Computational prediction of structurally related MEPs, including the MAX effector family, revealed their temporal co-regulation in the same co-expression modules. We characterized 32 MEP genes and demonstrate that Mep effectors are predominantly targeted to the cytoplasm of rice cells via the biotrophic interfacial complex and use a common unconventional secretory pathway. Taken together, our study reveals major changes in gene expression associated with blast disease and identifies a diverse repertoire of effectors critical for successful infection.

Original languageEnglish
Pages (from-to)1360-1385
Number of pages26
JournalPlant Cell
Volume35
Issue number5
Early online date18 Feb 2023
DOIs
Publication statusPublished - May 2023

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