The use of solubility, dialyzability, and Caco-2 cell methods to predict iron bioavailability

Susan Fairweather-Tait, Ian Phillips, Gary Wortley, Linda Harvey, Ray Glahn

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


An update on in vitro methods employed to assess iron bioavailability is presented. Solubility and dialyzability are not useful predictors of iron absorption, whereas Caco-2 cell models provide useful comparisons on the availability of iron from different sources. Strengths and weaknesses of in vitro approaches are briefly described, including the need to characterize Caco-2 cells in order to interpret results correctly. Further developments are required to refine Caco-2 model systems, including optimization and standardization of methods.
Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalInternational Journal for Vitamin and Nutrition Research
Issue number3
Publication statusPublished - May 2007


  • Biological Availability
  • Caco-2 Cells
  • Dialysis
  • Humans
  • Intestinal Absorption
  • Iron, Dietary
  • Models, Biological
  • Predictive Value of Tests
  • Solubility

Cite this